CBM UniTrap Project

UniTrap UNI18912

Trapped Gene	Tmem85 (ENSMUSG00000027131)
Vector Insertion	Chr 2: 112207674 - 112207975
Public Clones	not available
Private Clones	OST411084 (lexicon) OST276788 (lexicon) OST240838 (lexicon)
Severity of mutation (?)	Insertion after 16% of polypeptide chain

Suggested RT-PCR (?)

Control PCR for the trapped allele
Upstream Exon ENSMUSE00000166377 (Chr2:112207976..112208135 -)				Vector Sequence
Forward Primer	Location	Tm	%GC	LacZrt Reverse Primer	Location
CTTACCTGCAGAACGCAAGC	Chr2:112208102..112208121	61.1	55	TGGCGAAAGGGGGATGTG	Gene trap vector


Control PCR for the wild-type allele
Upstream Exon ENSMUSE00000166377 (Chr2:112207976..112208135 -)				Downstram Exon ENSMUSE00000166376 (Chr2:112207559..112207673 -)
Forward Primer	Location	Tm	%GC	Reverse Primer	Location	Tm	%GC
CTTACCTGCAGAACGCAAGC	Chr2:112208102..112208121	61.1	55	GGTCTCTTGCACGCTGGTAT	Chr2:112207558..112207577	60.28	55

Other possible Exon-Primers for RT-PCR (?)

	Exon ID	Exon location	Forward Primer	Primer location	Tm	%GC
upstream	ENSMUSE00000166377	Chr2:112207976..112208135	CTTACCTGCAGAACGCAAGC	Chr2:112208102..112208121	61.1	55
* Putative Vector Insertion (Chr 2: 112207674 - 112207975) *
	Exon ID	Exon location	Reverse Primer	Primer location	Tm	%GC
downstream	ENSMUSE00000166376	Chr2:112207559..112207673	GGTCTCTTGCACGCTGGTAT	Chr2:112207558..112207577	60.28	55
downstream	ENSMUSE00000166378	Chr2:112204309..112204462	TGGCCATAAGTGCTTGAATG	Chr2:112204296..112204315	59.69	45
downstream	ENSMUSE00000166379	Chr2:112203949..112204109	AAGGCTAGCCAGTCTGATGC	Chr2:112203943..112203962	59.6	55
downstream	ENSMUSE00000166380	Chr2:112203180..112203511	CTCACCACAGGAGGCTCTTC	Chr2:112203344..112203363	59.99	60

Suggested Genomic-PCR (?)

	Forward	Forward location	Tm	%GC	Reverse name	Reverse	Reverse Location
PCR 1	TCTCGAGTGAAGCTCGGTATC	Chr2:112207946..112207967	59.59	52.38	LacZ	CCAGGGTTTTCCCAGTCACG	Gene trap vector

Come back to gene ENSMUSG00000027131