Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI18948
Trapped Gene
Fuca1 (ENSMUSG00000028673)
Vector Insertion
Chr 4: 135492938 - 135494988
Public Clones not available
Private Clones OST409265 (lexicon) OST7107 (lexicon)
Severity of mutation (?) Insertion after 90% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000181221 (Chr4:135492838..135492937 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGGGATCGTGAACCTCAAAT Chr4:135492892..135492911 60.32 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000181221 (Chr4:135492838..135492937 +)
Downstram Exon
ENSMUSE00000341316 (Chr4:135494989..135495203 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGGGATCGTGAACCTCAAAT Chr4:135492892..135492911 60.32 45 AGAGTCCACGCAAACTCCAC Chr4:135495110..135495129 60.31 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000262007 Chr4:135476674..135477065 ATCAGTGGGCCGAACTCTTT Chr4:135477029..135477048 61.02 50
upstream ENSMUSE00000400130 Chr4:135478882..135479016 ACTGGAACTCGAAGGACGTG Chr4:135478947..135478966 60.3 55
upstream ENSMUSE00000181220 Chr4:135481464..135481601 ATACGCTACGGCCTCTACCA Chr4:135481468..135481487 59.75 55
upstream ENSMUSE00000181225 Chr4:135485814..135485919 ACTGGAACTCCACCAGCTTC Chr4:135485867..135485886 59.31 55
upstream ENSMUSE00000181219 Chr4:135488814..135489014 CTGGGGCTATCGAAAAGACA Chr4:135488954..135488973 60.21 50
upstream ENSMUSE00000181227 Chr4:135490605..135490795 AGAAAGGCTTCTTGCTGTCG Chr4:135490691..135490710 59.76 50
upstream ENSMUSE00000181221 Chr4:135492838..135492937 TGGGATCGTGAACCTCAAAT Chr4:135492892..135492911 60.32 45

*** Putative Vector Insertion (Chr 4: 135492938 - 135494988) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000341316 Chr4:135494989..135495203 AGAGTCCACGCAAACTCCAC Chr4:135495110..135495129 60.31 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AATGGGATCGTGAACCTCAA Chr4:135492891..135492911 60.32 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AATGGGATCGTGAACCTCAA Chr4:135492891..135492911 60.32 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000028673