Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI18982
Trapped Gene
Myl2 (ENSMUSG00000013936)
Vector Insertion
Chr 5: 122553945 - 122554835
Public Clones not available
Private Clones OST407758 (lexicon) OST17146 (lexicon)
Severity of mutation (?) Insertion after 55% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000538153 (Chr5:122553840..122553944 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000538153 (Chr5:122553840..122553944 +)
Downstram Exon
ENSMUSE00000189067 (Chr5:122554836..122554914 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000688851 Chr5:122550966..122551038 No primer for this exon
upstream ENSMUSE00000688867 Chr5:122550989..122551038 No primer for this exon
upstream ENSMUSE00000688853 Chr5:122550992..122551038 No primer for this exon
upstream ENSMUSE00000222492 Chr5:122551758..122551847 No primer for this exon
upstream ENSMUSE00000538154 Chr5:122552705..122552780 No primer for this exon
upstream ENSMUSE00000538153 Chr5:122553840..122553944 No primer for this exon

*** Putative Vector Insertion (Chr 5: 122553945 - 122554835) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000189067 Chr5:122554836..122554914 No primer for this exon
downstream ENSMUSE00000222472 Chr5:122554997..122555045 No primer for this exon
downstream ENSMUSE00000688850 Chr5:122554997..122555302 No primer for this exon
downstream ENSMUSE00000222465 Chr5:122556680..122556798 No primer for this exon
downstream ENSMUSE00000688854 Chr5:122556680..122556863 No primer for this exon
downstream ENSMUSE00000688852 Chr5:122563158..122563481 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACCGTGTTCCTCACGATGTT Chr5:122553909..122553929 60.43 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACCGTGTTCCTCACGATGTT Chr5:122553909..122553929 60.43 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000013936