Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI19249
Trapped Gene
Slc25a38 (ENSMUSG00000032519)
Vector Insertion
Chr 9: 120019691 - 120025619
Public Clones not available
Private Clones OST398576 (lexicon)
Severity of mutation (?) Insertion after 13% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000351566 (Chr9:120019517..120019690 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGCAGTCGCAGGATGTAGAG Chr9:120019650..120019669 60.16 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000351566 (Chr9:120019517..120019690 +)
Downstram Exon
ENSMUSE00000528232 (Chr9:120025620..120025741 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGCAGTCGCAGGATGTAGAG Chr9:120019650..120019669 60.16 55 CAGACGGGTTTTGAGGAGAT Chr9:120025718..120025737 59.14 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000351566 Chr9:120019517..120019690 TGCAGTCGCAGGATGTAGAG Chr9:120019650..120019669 60.16 55

*** Putative Vector Insertion (Chr 9: 120019691 - 120025619) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000528232 Chr9:120025620..120025741 CAGACGGGTTTTGAGGAGAT Chr9:120025718..120025737 59.14 50
downstream ENSMUSE00000372567 Chr9:120026571..120026655 GGAGACTTTCTGTGCGAACC Chr9:120026632..120026651 59.85 55
downstream ENSMUSE00000220423 Chr9:120029375..120029554 TGATGGGTGACATGCAGACT Chr9:120029534..120029553 60.12 50
downstream ENSMUSE00000220417 Chr9:120029791..120029965 ATCACGGAGGAGAGTTGCTG Chr9:120029901..120029920 60.41 55
downstream ENSMUSE00000220420 Chr9:120031212..120031378 TCACGTCTGCAGGTTGAGTC Chr9:120031310..120031329 60.03 55
downstream ENSMUSE00000353169 Chr9:120032760..120033435 AGAGTAGGCAGGTGGCTGAA Chr9:120033165..120033184 60.01 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TGTAGAGGACACGGTGGAGA Chr9:120022664..120022684 59.26 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGTAGAGGACACGGTGGAGA Chr9:120022664..120022684 59.26 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000032519