Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI19344
Trapped Gene
Mvp (ENSMUSG00000030681)
Vector Insertion
Chr 7: 134145473 - 134158032
Public Clones CMHD-GT_520E12-5S (cmhd) CMHD-GT_520D11-5S (cmhd) IST12906D2 (tigm)
IST14608B7 (tigm) IST13694E7 (tigm) IST14551H10 (tigm) IST12906D2 (tigm)
IST13694E7 (tigm)
Private Clones OST394032 (lexicon) OST286265 (lexicon) OST284897 (lexicon) OST250860 (lexicon)
OST183843 (lexicon) OST109313 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000416335 (Chr7:134158033..134158108 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GTTGGTGAGGGAAATTCCTG Chr7:134158069..134158088 59.38 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000416335 (Chr7:134158033..134158108 -)
Downstram Exon
ENSMUSE00000416328 (Chr7:134145323..134145472 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GTTGGTGAGGGAAATTCCTG Chr7:134158069..134158088 59.38 50 CAACCTCTACACGGGACACA Chr7:134145335..134145354 59.59 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000416335 Chr7:134158033..134158108 GTTGGTGAGGGAAATTCCTG Chr7:134158069..134158088 59.38 50

*** Putative Vector Insertion (Chr 7: 134145473 - 134158032) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000416328 Chr7:134145323..134145472 CAACCTCTACACGGGACACA Chr7:134145335..134145354 59.59 55
downstream ENSMUSE00000201918 Chr7:134144994..134145189 GACACAGGGTTGGCCACTAT Chr7:134145096..134145115 59.85 55
downstream ENSMUSE00000201929 Chr7:134141987..134142110 GTCCAGCAATGCCTTCAGAT Chr7:134142026..134142045 60.23 50
downstream ENSMUSE00000201920 Chr7:134141740..134141871 GTTTGATGACTGTGGCCTGA Chr7:134141787..134141806 59.68 50
downstream ENSMUSE00000201925 Chr7:134139752..134139846 TGTAAGGATCACAGCGTCCA Chr7:134139736..134139755 60.26 50
downstream ENSMUSE00000201922 Chr7:134139250..134139486 CACCGTCACTAGCCATTCCT Chr7:134139378..134139397 60.13 55
downstream ENSMUSE00000201917 Chr7:134137034..134137315 AGGGATAGCCTGACGCTCTT Chr7:134137060..134137079 60.37 55
downstream ENSMUSE00000201921 Chr7:134136068..134136312 TAGCTGACCACTCGGGTCTT Chr7:134136103..134136122 59.87 55
downstream ENSMUSE00000201924 Chr7:134135789..134135986 CAGTTTCGATGGTGATGACG Chr7:134135807..134135826 60.11 50
downstream ENSMUSE00000201923 Chr7:134133060..134133446 AATTTCGATGGCTAGCTGGA Chr7:134133067..134133086 59.81 45
downstream ENSMUSE00000201926 Chr7:134132340..134132456 TCAGCTTCTGACTGGTCCAA Chr7:134132357..134132376 59.54 50
downstream ENSMUSE00000291837 Chr7:134131832..134131958 GAGCCTTCTCCCTCAATCCT Chr7:134131856..134131875 59.78 55
downstream ENSMUSE00000201927 Chr7:134130991..134131179 GCTCCATCTCTCGCACTTTC Chr7:134131115..134131134 60.1 55
downstream ENSMUSE00000519861 Chr7:134130383..134130628 ACGAGCCATCTGTGATGAGA Chr7:134130552..134130571 59.37 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCCACAATGGCCTATTTGAA Chr7:134154982..134155002 60.84 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCCACAATGGCCTATTTGAA Chr7:134154982..134155002 60.84 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 AGTGACCCCGAATGACTCAC Chr7:134155059..134155079 59.97 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 AGGAATGGTCCCAGTGATGA Chr7:134155083..134155103 60.33 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000030681