Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI19444
Trapped Gene
Nav1 (ENSMUSG00000009418)
Vector Insertion
Chr 1: 137504482 - 137584495
Public Clones (ggtc) (ggtc) IST12814C6 (tigm) IST13082G2 (tigm) IST12144G5 (tigm)
IST11330B5 (tigm) IST14539H6 (tigm) IST13562D8 (tigm) IST14226D2 (tigm)
IST13873C4 (tigm) IST14012D2 (tigm) IST14429D12 (tigm)
Private Clones OST391034 (lexicon) OST370207 (lexicon) OST274167 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000690480 (Chr1:137584496..137584682 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000690480 (Chr1:137584496..137584682 -)
Downstram Exon
ENSMUSE00000690479 (Chr1:137503024..137504481 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000690480 Chr1:137584496..137584682 No primer for this exon

*** Putative Vector Insertion (Chr 1: 137504482 - 137584495) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000429775 Chr1:137503739..137503872 No primer for this exon
downstream ENSMUSE00000690479 Chr1:137503024..137504481 No primer for this exon
downstream ENSMUSE00000429766 Chr1:137499122..137499225 No primer for this exon
downstream ENSMUSE00000239238 Chr1:137481132..137482286 No primer for this exon
downstream ENSMUSE00000401133 Chr1:137481132..137481932 No primer for this exon
downstream ENSMUSE00000239228 Chr1:137433774..137433876 No primer for this exon
downstream ENSMUSE00000369434 Chr1:137428926..137429291 No primer for this exon
downstream ENSMUSE00000342073 Chr1:137369310..137369448 No primer for this exon
downstream ENSMUSE00000387979 Chr1:137368736..137369033 No primer for this exon
downstream ENSMUSE00000240534 Chr1:137367057..137367747 No primer for this exon
downstream ENSMUSE00000240525 Chr1:137366209..137366643 No primer for this exon
downstream ENSMUSE00000240517 Chr1:137365433..137365474 No primer for this exon
downstream ENSMUSE00000240583 Chr1:137364218..137364366 No primer for this exon
downstream ENSMUSE00000240578 Chr1:137362416..137362586 No primer for this exon
downstream ENSMUSE00000240653 Chr1:137361283..137361335 No primer for this exon
downstream ENSMUSE00000240572 Chr1:137360492..137360515 No primer for this exon
downstream ENSMUSE00000240644 Chr1:137360299..137360376 No primer for this exon
downstream ENSMUSE00000240632 Chr1:137357268..137357351 No primer for this exon
downstream ENSMUSE00000240623 Chr1:137356921..137357032 No primer for this exon
downstream ENSMUSE00000240618 Chr1:137355233..137355354 No primer for this exon
downstream ENSMUSE00000690482 Chr1:137354438..137354446 No primer for this exon
downstream ENSMUSE00000240613 Chr1:137351711..137351907 No primer for this exon
downstream ENSMUSE00000334951 Chr1:137351268..137351460 No primer for this exon
downstream ENSMUSE00000158909 Chr1:137351011..137351179 No primer for this exon
downstream ENSMUSE00000158910 Chr1:137350622..137350719 No primer for this exon
downstream ENSMUSE00000158916 Chr1:137349770..137349865 No primer for this exon
downstream ENSMUSE00000158920 Chr1:137349382..137349541 No primer for this exon
downstream ENSMUSE00000158907 Chr1:137348749..137348984 No primer for this exon
downstream ENSMUSE00000158908 Chr1:137347363..137347517 No primer for this exon
downstream ENSMUSE00000158906 Chr1:137347147..137347218 No primer for this exon
downstream ENSMUSE00000158913 Chr1:137346472..137346668 No primer for this exon
downstream ENSMUSE00000158918 Chr1:137345508..137345626 No primer for this exon
downstream ENSMUSE00000240675 Chr1:137340211..137340408 No primer for this exon
downstream ENSMUSE00000428972 Chr1:137338261..137338356 No primer for this exon
downstream ENSMUSE00000596238 Chr1:137335450..137338356 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AATGACACTGGAGGGACAGC Chr1:137524517..137524537 60.12 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AATGACACTGGAGGGACAGC Chr1:137524517..137524537 60.12 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 GTGGAAGGGAGAGGACCCTA Chr1:137539630..137539650 60.45 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 TCTCCAAGGGCATTTTAGCA Chr1:137539640..137539660 60.71 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000009418