Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI19446
Trapped Gene
Mrpl52 (ENSMUSG00000010406)
Vector Insertion
Chr 14: 55045929 - 55046007
Public Clones IST10655G6 (tigm) IST13138A2 (tigm)
Private Clones OST391014 (lexicon)
Severity of mutation (?) Insertion after 24% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000124125 (Chr14:55045871..55045928 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000124125 (Chr14:55045871..55045928 +)
Downstram Exon
ENSMUSE00000124127 (Chr14:55046008..55046075 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000124124 Chr14:55045747..55045797 No primer for this exon
upstream ENSMUSE00000124125 Chr14:55045871..55045928 No primer for this exon

*** Putative Vector Insertion (Chr 14: 55045929 - 55046007) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000124127 Chr14:55046008..55046075 No primer for this exon
downstream ENSMUSE00000124126 Chr14:55047421..55047485 No primer for this exon
downstream ENSMUSE00000124123 Chr14:55048419..55048590 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAATCGCCTTGCAGCACATC Chr14:55045980..55046000 62.25 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGTGGCGACTCCAGTGAGTG Chr14:55045917..55045937 61.5 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000010406