Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI19590
Trapped Gene
Aqp11 (ENSMUSG00000042797)
Vector Insertion
Chr 7: 104877598 - 104885878
Public Clones not available
Private Clones OST388295 (lexicon)
Severity of mutation (?) Insertion after 89% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000346173 (Chr7:104885879..104886497 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TAGCTTGCAGGAATCCCATC Chr7:104886021..104886040 60.18 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000346173 (Chr7:104885879..104886497 -)
Downstram Exon
ENSMUSE00000388766 (Chr7:104877481..104877597 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TAGCTTGCAGGAATCCCATC Chr7:104886021..104886040 60.18 50 AAAGCACGGAAAGTGCAGAG Chr7:104877511..104877530 60.57 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000346173 Chr7:104885879..104886497 TAGCTTGCAGGAATCCCATC Chr7:104886021..104886040 60.18 50

*** Putative Vector Insertion (Chr 7: 104877598 - 104885878) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000388766 Chr7:104877481..104877597 AAAGCACGGAAAGTGCAGAG Chr7:104877511..104877530 60.57 50
downstream ENSMUSE00000343233 Chr7:104874889..104875214 GTTCGAGTCTTTGGGAGTGG Chr7:104875093..104875112 59.7 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGATAATCGCCTTGCAGCAC Chr7:104885810..104885830 60.38 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGAGCCTCTATGACCAGACG Chr7:104879825..104879845 59.83 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000042797