Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI19650
Trapped Gene
2410131K14Rik (ENSMUSG00000032840)
Vector Insertion
Chr 5: 118705836 - 118708984
Public Clones not available
Private Clones OST384982 (lexicon)
Severity of mutation (?) Insertion after 44% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000290475 (Chr5:118705679..118705835 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CCAAGGAAAACACCTCCTCA Chr5:118705804..118705823 60.08 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000290475 (Chr5:118705679..118705835 +)
Downstram Exon
ENSMUSE00000290467 (Chr5:118708985..118709136 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CCAAGGAAAACACCTCCTCA Chr5:118705804..118705823 60.08 50 CGCAGTACTCGTAGGCTTCA Chr5:118709113..118709132 59.24 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000344235 Chr5:118695278..118695467 TTCGGCCTCTCGCTAGTTTA Chr5:118695423..118695442 60.11 50
upstream ENSMUSE00000290475 Chr5:118705679..118705835 CCAAGGAAAACACCTCCTCA Chr5:118705804..118705823 60.08 50

*** Putative Vector Insertion (Chr 5: 118705836 - 118708984) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000290467 Chr5:118708985..118709136 CGCAGTACTCGTAGGCTTCA Chr5:118709113..118709132 59.24 55
downstream ENSMUSE00000290455 Chr5:118709384..118709497 GTGGTCTTCAACTGCCATGA Chr5:118709461..118709480 59.68 50
downstream ENSMUSE00000459574 Chr5:118711109..118713113 AAGCACGAGCAGACTGGATT Chr5:118712886..118712905 60.02 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTGAAGCCAGCCCTAGACAC Chr5:118705840..118705860 59.87 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GTGAAGCCAGCCCTAGACAC Chr5:118705840..118705860 59.87 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000032840