Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI19986
Trapped Gene
Mapk1ip1 (ENSMUSG00000041775)
Vector Insertion
Chr 7: 146027530 - 146028610
Public Clones not available
Private Clones OST375810 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000714995 (Chr7:146027561..146028609 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TCCCCGAATAATCCTTACCC Chr7:146027934..146027953 59.98 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000714995 (Chr7:146027561..146028609 -)
Downstram Exon
ENSMUSE00000468144 (Chr7:146027531..146028713 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TCCCCGAATAATCCTTACCC Chr7:146027934..146027953 59.98 50 AGGACCAACTGGATCTGTGG Chr7:146028154..146028173 59.96 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000470206 Chr7:146037640..146037943 CGACGCCCTCTACGTTCTTA Chr7:146037812..146037831 60.4 55
upstream ENSMUSE00000710036 Chr7:146037640..146037940 CGACGCCCTCTACGTTCTTA Chr7:146037812..146037831 60.4 55
upstream ENSMUSE00000719338 Chr7:146037640..146037956 CGACGCCCTCTACGTTCTTA Chr7:146037812..146037831 60.4 55
upstream ENSMUSE00000716184 Chr7:146032260..146032617 TAGCGCTGACCTTTGACCTT Chr7:146032322..146032341 60.01 50
upstream ENSMUSE00000714995 Chr7:146027561..146028609 TCCCCGAATAATCCTTACCC Chr7:146027934..146027953 59.98 50
upstream ENSMUSE00000468144 Chr7:146027531..146028713 TCCCCGAATAATCCTTACCC Chr7:146027934..146027953 59.98 50

*** Putative Vector Insertion (Chr 7: 146027530 - 146028610) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000711209 Chr7:146027506..146028713 AGGACCAACTGGATCTGTGG Chr7:146028154..146028173 59.96 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000041775