Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI20021
Trapped Gene
Dsn1 (ENSMUSG00000027635)
Vector Insertion
Chr 2: 156831583 - 156831610
Public Clones not available
Private Clones OST374878 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000661350 (Chr2:156831584..156832098 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TAGTGAGGCCCCTGTGAGAC Chr2:156831659..156831678 60.26 60 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000661350 (Chr2:156831584..156832098 -)
Downstram Exon
ENSMUSE00000661346 (Chr2:156831584..156831609 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TAGTGAGGCCCCTGTGAGAC Chr2:156831659..156831678 60.26 60 No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000661347 Chr2:156832711..156832811 GCGGGAGTTTCGTAAGTAGC Chr2:156832783..156832802 59 55
upstream ENSMUSE00000661351 Chr2:156832711..156832890 GCGGGAGTTTCGTAAGTAGC Chr2:156832783..156832802 59 55
upstream ENSMUSE00000661346 Chr2:156831584..156831609 No primer for this exon
upstream ENSMUSE00000661350 Chr2:156831584..156832098 TAGTGAGGCCCCTGTGAGAC Chr2:156831659..156831678 60.26 60
upstream ENSMUSE00000713733 Chr2:156831584..156831609 No primer for this exon

*** Putative Vector Insertion (Chr 2: 156831583 - 156831610) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000661349 Chr2:156830904..156831227 TGCGTCATCATTTCCTGGTA Chr2:156831100..156831119 60.07 45
downstream ENSMUSE00000171369 Chr2:156828425..156828498 TGGCTCTGGGCTAACTTCAC Chr2:156828437..156828456 60.4 55
downstream ENSMUSE00000171366 Chr2:156827419..156827491 No primer for this exon
downstream ENSMUSE00000171368 Chr2:156826570..156826660 TGCAGTGTTCCATCATTTCC Chr2:156826575..156826594 59.5 45
downstream ENSMUSE00000171359 Chr2:156824872..156824931 TGCTTCCAGTGAAAAATCTGC Chr2:156824882..156824902 60.38 42.86
downstream ENSMUSE00000171367 Chr2:156824456..156824533 GGTGGAACCTCGTTCTGGTA Chr2:156824449..156824468 59.97 55
downstream ENSMUSE00000171363 Chr2:156823343..156823490 CTGCAGGATCCACCTTGACT Chr2:156823422..156823441 60.26 55
downstream ENSMUSE00000171364 Chr2:156822469..156822556 CTTGCAGCTGCTTCACTGAT Chr2:156822498..156822517 59.34 50
downstream ENSMUSE00000661348 Chr2:156821013..156822017 TTTCCGAGCAGGTGAAGAGT Chr2:156821952..156821971 59.99 50
downstream ENSMUSE00000639505 Chr2:156821001..156822017 TTTCCGAGCAGGTGAAGAGT Chr2:156821952..156821971 59.99 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGATGACGTCAGTGACCAGA Chr2:156831589..156831609 59.67 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACCAGATCAGAAGGCGTGAC Chr2:156831575..156831595 60.27 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000027635