Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI20090
Trapped Gene
Def8 (ENSMUSG00000001482)
Vector Insertion
Chr 8: 125971687 - 125971813
Public Clones not available
Private Clones OST373173 (lexicon) OST303303 (lexicon) OST297769 (lexicon) OST297403 (lexicon)
OST207829 (lexicon) OST130081 (lexicon) OST104542 (lexicon) OST75945 (lexicon)
OST69001 (lexicon) OST51474 (lexicon)
Severity of mutation (?) Insertion after 2% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000720527 (Chr8:125971688..125971812 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000720527 (Chr8:125971688..125971812 +)
Downstram Exon
ENSMUSE00000487010 (Chr8:125971688..125971812 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000362720 Chr8:125966886..125966951 No primer for this exon
upstream ENSMUSE00000706215 Chr8:125966896..125966951 No primer for this exon
upstream ENSMUSE00000605685 Chr8:125966905..125966951 No primer for this exon
upstream ENSMUSE00000678175 Chr8:125967208..125967345 No primer for this exon
upstream ENSMUSE00000712640 Chr8:125969998..125970084 No primer for this exon
upstream ENSMUSE00000722052 Chr8:125969998..125970084 No primer for this exon
upstream ENSMUSE00000678199 Chr8:125970059..125970084 No primer for this exon
upstream ENSMUSE00000678176 Chr8:125971217..125971263 No primer for this exon

*** Putative Vector Insertion (Chr 8: 125971687 - 125971813) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000487010 Chr8:125971688..125971812 No primer for this exon
downstream ENSMUSE00000502574 Chr8:125971688..125971812 No primer for this exon
downstream ENSMUSE00000720527 Chr8:125971688..125971812 No primer for this exon
downstream ENSMUSE00000287889 Chr8:125973201..125973298 No primer for this exon
downstream ENSMUSE00000215300 Chr8:125976645..125976794 No primer for this exon
downstream ENSMUSE00000215307 Chr8:125978129..125978270 No primer for this exon
downstream ENSMUSE00000215306 Chr8:125979314..125979478 No primer for this exon
downstream ENSMUSE00000215314 Chr8:125980306..125980433 No primer for this exon
downstream ENSMUSE00000215311 Chr8:125980571..125980684 No primer for this exon
downstream ENSMUSE00000215313 Chr8:125982251..125982331 No primer for this exon
downstream ENSMUSE00000287705 Chr8:125983417..125983557 No primer for this exon
downstream ENSMUSE00000287678 Chr8:125983717..125983826 No primer for this exon
downstream ENSMUSE00000678174 Chr8:125983717..125984415 No primer for this exon
downstream ENSMUSE00000401218 Chr8:125985364..125987162 No primer for this exon
downstream ENSMUSE00000434262 Chr8:125985364..125987161 No primer for this exon
downstream ENSMUSE00000605684 Chr8:125985364..125987170 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GAGCCTGGGACACTCATCTC Chr8:125971649..125971669 59.8 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAGCCTGGGACACTCATCTC Chr8:125971649..125971669 59.8 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000001482