Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI20149
Trapped Gene
Calcoco2 (ENSMUSG00000006056)
Vector Insertion
Chr 11: 95964665 - 95964771
Public Clones not available
Private Clones OST371357 (lexicon) OST251620 (lexicon) OST215499 (lexicon) OST182833 (lexicon)
OST170485 (lexicon) OST361 (lexicon)
Severity of mutation (?) Insertion after 38% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000110945 (Chr11:95964772..95964874 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000110945 (Chr11:95964772..95964874 -)
Downstram Exon
ENSMUSE00000110950 (Chr11:95964531..95964664 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000674120 Chr11:95973251..95973276 No primer for this exon
upstream ENSMUSE00000380218 Chr11:95968745..95968918 No primer for this exon
upstream ENSMUSE00000110945 Chr11:95964772..95964874 No primer for this exon

*** Putative Vector Insertion (Chr 11: 95964665 - 95964771) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000110950 Chr11:95964531..95964664 No primer for this exon
downstream ENSMUSE00000110948 Chr11:95963962..95964066 No primer for this exon
downstream ENSMUSE00000110946 Chr11:95961648..95961716 No primer for this exon
downstream ENSMUSE00000674112 Chr11:95961581..95961646 No primer for this exon
downstream ENSMUSE00000674118 Chr11:95961561..95961716 No primer for this exon
downstream ENSMUSE00000390101 Chr11:95961228..95961716 No primer for this exon
downstream ENSMUSE00000674117 Chr11:95960640..95961266 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGACTGGGATAATCGCCTTG Chr11:95964709..95964729 59.15 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACTGGGTCGAAGATGCTGAT Chr11:95964740..95964760 59.69 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000006056