Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI20383
Trapped Gene
Zmat5 (ENSMUSG00000009076)
Vector Insertion
Chr 11: 4628651 - 4630189
Public Clones not available
Private Clones OST364948 (lexicon) OST255672 (lexicon) OST221468 (lexicon) OST217881 (lexicon)
OST183577 (lexicon) OST182600 (lexicon)
Severity of mutation (?) Insertion after 37% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000104281 (Chr11:4628588..4628650 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000104281 (Chr11:4628588..4628650 +)
Downstram Exon
ENSMUSE00000104286 (Chr11:4630190..4630270 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000104283 Chr11:4604688..4604724 No primer for this exon
upstream ENSMUSE00000104287 Chr11:4622361..4622514 No primer for this exon
upstream ENSMUSE00000104281 Chr11:4628588..4628650 No primer for this exon

*** Putative Vector Insertion (Chr 11: 4628651 - 4630189) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000104286 Chr11:4630190..4630270 No primer for this exon
downstream ENSMUSE00000104279 Chr11:4632070..4632181 No primer for this exon
downstream ENSMUSE00000442230 Chr11:4637335..4637535 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTGGCCTACTAATCGCCTTG Chr11:4628693..4628713 59.86 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTACCGTGACTGGGAAAACC Chr11:4628698..4628718 59.45 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000009076