Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI20551
Trapped Gene
Mrpl2 (ENSMUSG00000002767)
Vector Insertion
Chr 17: 46786832 - 46787082
Public Clones CMHD-GT_543F4-3 (cmhd)
Private Clones OST358973 (lexicon) OST128823 (lexicon) OST71346 (lexicon) OST31829 (lexicon)
OST29837 (lexicon) OST24708 (lexicon) OST18515 (lexicon) OST10927 (lexicon)
Severity of mutation (?) Insertion after 77% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000695542 (Chr17:46786833..46787088 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000695542 (Chr17:46786833..46787088 +)
Downstram Exon
ENSMUSE00000412005 (Chr17:46786833..46787081 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000350685 Chr17:46783197..46783347 No primer for this exon
upstream ENSMUSE00000695541 Chr17:46783199..46783347 No primer for this exon
upstream ENSMUSE00000695544 Chr17:46783219..46783347 No primer for this exon
upstream ENSMUSE00000136809 Chr17:46784339..46784507 No primer for this exon
upstream ENSMUSE00000695543 Chr17:46784345..46784507 No primer for this exon
upstream ENSMUSE00000542551 Chr17:46785186..46785327 No primer for this exon
upstream ENSMUSE00000542549 Chr17:46785421..46785536 No primer for this exon
upstream ENSMUSE00000136810 Chr17:46785608..46785718 No primer for this exon
upstream ENSMUSE00000695540 Chr17:46785619..46785718 No primer for this exon
upstream ENSMUSE00000136807 Chr17:46785984..46786057 No primer for this exon
upstream ENSMUSE00000695538 Chr17:46785984..46786057 No primer for this exon

*** Putative Vector Insertion (Chr 17: 46786832 - 46787082) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000412005 Chr17:46786833..46787081 No primer for this exon
downstream ENSMUSE00000695537 Chr17:46786833..46787080 No primer for this exon
downstream ENSMUSE00000695542 Chr17:46786833..46787088 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACTGCAACTGTAGGCCGAGT Chr17:46786849..46786869 59.94 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACTGCAACTGTAGGCCGAGT Chr17:46786849..46786869 59.94 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000002767