Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI20746
Trapped Gene
Vangl1 (ENSMUSG00000027860)
Vector Insertion
Chr 3: 101993471 - 102000714
Public Clones not available
Private Clones OST353180 (lexicon)
Severity of mutation (?) Insertion after 5% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000453884 (Chr3:102000715..102000899 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ATCAGACGAAGCTTGCCATT Chr3:102000789..102000808 59.84 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000453884 (Chr3:102000715..102000899 -)
Downstram Exon
ENSMUSE00000453879 (Chr3:101993332..101993470 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ATCAGACGAAGCTTGCCATT Chr3:102000789..102000808 59.84 45 GCCTGGATGGTGACAGACTT Chr3:101993371..101993390 60.12 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000453892 Chr3:102008435..102008531 No primer for this exon
upstream ENSMUSE00000453884 Chr3:102000715..102000899 ATCAGACGAAGCTTGCCATT Chr3:102000789..102000808 59.84 45

*** Putative Vector Insertion (Chr 3: 101993471 - 102000714) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000453879 Chr3:101993332..101993470 GCCTGGATGGTGACAGACTT Chr3:101993371..101993390 60.12 55
downstream ENSMUSE00000453875 Chr3:101987874..101988481 AGACTGCGTATTGCACGATG Chr3:101987991..101988010 59.9 50
downstream ENSMUSE00000370676 Chr3:101970761..101970894 CTCGGAATTTGGATGCTGTT Chr3:101970781..101970800 60.07 45
downstream ENSMUSE00000173686 Chr3:101969278..101969410 TACAACTCGTTGTGGCTGGA Chr3:101969304..101969323 60.3 50
downstream ENSMUSE00000173682 Chr3:101967222..101967456 CATGCTGTGGTAGTGCTGCT Chr3:101967254..101967273 60.08 55
downstream ENSMUSE00000396395 Chr3:101962119..101962420 GAGCCATCGATCCTTGTCAT Chr3:101962336..101962355 60.04 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TGTAGGTAATCGCCTTGCAG Chr3:101994649..101994669 58.94 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CGAGGACTCTGAGCCTGTTT Chr3:101994699..101994719 59.6 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000027860