Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI20807
Trapped Gene
Gtf2f1 (
ENSMUSG00000002658
)
Vector Insertion
Chr 17: 57147330 - 57149110
Public Clones
not available
Private Clones
OST351704 (lexicon)
Severity of mutation
(?)
Insertion after 19% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000657110 (Chr17:57149111..57149183 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000657110 (Chr17:57149111..57149183 -)
Downstram Exon
ENSMUSE00000657109 (Chr17:57147136..57147329 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000505928
Chr17:57150532..57150841
No primer for this exon
upstream
ENSMUSE00000657111
Chr17:57150399..57150445
No primer for this exon
upstream
ENSMUSE00000657110
Chr17:57149111..57149183
No primer for this exon
*** Putative Vector Insertion (Chr 17: 57147330 - 57149110) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000657109
Chr17:57147136..57147329
No primer for this exon
downstream
ENSMUSE00000657108
Chr17:57146409..57146579
No primer for this exon
downstream
ENSMUSE00000417463
Chr17:57144856..57144967
No primer for this exon
downstream
ENSMUSE00000496736
Chr17:57144783..57144851
No primer for this exon
downstream
ENSMUSE00000501927
Chr17:57144783..57144967
No primer for this exon
downstream
ENSMUSE00000500083
Chr17:57144089..57144242
No primer for this exon
downstream
ENSMUSE00000541333
Chr17:57144089..57144242
No primer for this exon
downstream
ENSMUSE00000139846
Chr17:57143934..57143995
No primer for this exon
downstream
ENSMUSE00000606631
Chr17:57143934..57143995
No primer for this exon
downstream
ENSMUSE00000139852
Chr17:57143732..57143851
No primer for this exon
downstream
ENSMUSE00000606630
Chr17:57143732..57143851
No primer for this exon
downstream
ENSMUSE00000139854
Chr17:57143545..57143618
No primer for this exon
downstream
ENSMUSE00000606629
Chr17:57143545..57143618
No primer for this exon
downstream
ENSMUSE00000139847
Chr17:57143338..57143476
No primer for this exon
downstream
ENSMUSE00000606628
Chr17:57143338..57143476
No primer for this exon
downstream
ENSMUSE00000139857
Chr17:57143144..57143234
No primer for this exon
downstream
ENSMUSE00000606627
Chr17:57143144..57143234
No primer for this exon
downstream
ENSMUSE00000483281
Chr17:57142828..57143054
No primer for this exon
downstream
ENSMUSE00000538820
Chr17:57142828..57143054
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
CCTAACCCTGGGCTGGTAAT
Chr17:57149055..57149075
60.2
55
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
GGAACCAGGTAAGTGGGTGAT
Chr17:57149096..57149117
60.1
52.38
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000002658
