Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21159
Trapped Gene
Mark2 (ENSMUSG00000024969)
Vector Insertion
Chr 19: 7361869 - 7364805
Public Clones (sanger) (sanger)
Private Clones OST339768 (lexicon) OST299745 (lexicon)
Severity of mutation (?) Insertion after 8% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000146730 (Chr19:7364806..7364859 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ATCGACAAGACCCAGCTGAA Chr19:7364825..7364844 60.8 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000146730 (Chr19:7364806..7364859 -)
Downstram Exon
ENSMUSE00000146723 (Chr19:7361820..7361868 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ATCGACAAGACCCAGCTGAA Chr19:7364825..7364844 60.8 50 TGGGATGATTCAAAACCTTCA Chr19:7361804..7361824 60.29 38.1

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000695304 Chr19:7415658..7416334 AACCTCGGTCTTCGGAATCT Chr19:7416262..7416281 60.07 50
upstream ENSMUSE00000695218 Chr19:7369528..7369927 TGGAAGTCGCTGGTAGTCCT Chr19:7369547..7369566 59.87 55
upstream ENSMUSE00000695217 Chr19:7365093..7365272 ACCGGCTCCTTAAGACCATT Chr19:7365150..7365169 59.96 50
upstream ENSMUSE00000695301 Chr19:7365093..7365272 ACCGGCTCCTTAAGACCATT Chr19:7365150..7365169 59.96 50
upstream ENSMUSE00000146730 Chr19:7364806..7364859 ATCGACAAGACCCAGCTGAA Chr19:7364825..7364844 60.8 50

*** Putative Vector Insertion (Chr 19: 7361869 - 7364805) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000146723 Chr19:7361820..7361868 TGGGATGATTCAAAACCTTCA Chr19:7361804..7361824 60.29 38.1
downstream ENSMUSE00000146738 Chr19:7361491..7361556 CCACTGGCATACTCCATGAC Chr19:7361471..7361490 58.96 55
downstream ENSMUSE00000146735 Chr19:7361324..7361394 TTGGCTCGAGCTTCTTTTTC Chr19:7361312..7361331 59.71 45
downstream ENSMUSE00000146737 Chr19:7361059..7361115 TGACAGTACTGCACAGCAGACA Chr19:7361068..7361089 60.72 50
downstream ENSMUSE00000146736 Chr19:7360222..7360458 CCAAAGGTGAATTCGTTGCT Chr19:7360360..7360379 60.11 45
downstream ENSMUSE00000146729 Chr19:7359359..7359478 TACGGTATTTCCCCCTCAGT Chr19:7359420..7359439 58.38 50
downstream ENSMUSE00000695224 Chr19:7359177..7359249 CACGTTCATCCACCGATCTT Chr19:7359198..7359217 60.92 50
downstream ENSMUSE00000146726 Chr19:7359150..7359249 CACGTTCATCCACCGATCTT Chr19:7359198..7359217 60.92 50
downstream ENSMUSE00000486614 Chr19:7359043..7359089 CCCATTGACACCATCAACTC Chr19:7359027..7359046 58.77 50
downstream ENSMUSE00000550698 Chr19:7358955..7359067 CTGGATCTCTTCCCGTGTGT Chr19:7359005..7359024 60.11 55
downstream ENSMUSE00000550711 Chr19:7358955..7359038 CGTTGTACCTCTGGCCTACC Chr19:7358977..7358996 59.62 60
downstream ENSMUSE00000550710 Chr19:7357926..7358058 GGGGCTTCAAAGTGATGGTA Chr19:7358006..7358025 59.93 50
downstream ENSMUSE00000550709 Chr19:7357523..7357695 CCGCTTATTTTCTGCGTTGT Chr19:7357603..7357622 60.26 45
downstream ENSMUSE00000550708 Chr19:7357216..7357313 AAAAGTGGGGAGTTCCTGCT Chr19:7357242..7357261 60.11 50
downstream ENSMUSE00000550707 Chr19:7356288..7356449 CCGAGGCACCTCACAGTTAC Chr19:7356271..7356290 60.71 60
downstream ENSMUSE00000550706 Chr19:7355488..7355745 AAGGTGCTTCGACTGGACAC Chr19:7355610..7355629 60.31 55
downstream ENSMUSE00000643494 Chr19:7354476..7354502 TCTGGCAAACCTGAAAGACA Chr19:7354456..7354475 59.42 45
downstream ENSMUSE00000550704 Chr19:7352532..7352576 TCTCCACTCGGTCTTTGCTT Chr19:7352517..7352536 59.99 50
downstream ENSMUSE00000550701 Chr19:7349886..7351940 GATCTCCCGCATCATCTCAT Chr19:7351783..7351802 60 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CGACAAGACCCAGCTGAACT Chr19:7364821..7364841 60.44 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CGACAAGACCCAGCTGAACT Chr19:7364821..7364841 60.44 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 CATGAAATGAGCCCATGTGT Chr19:7364879..7364899 59.37 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 ACATAGGCGTGACTGGGAAA Chr19:7361804..7361824 60.52 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024969