Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21249
Trapped Gene
Vps54 (ENSMUSG00000020128)
Vector Insertion
Chr 11: 21171742 - 21175000
Public Clones IST12770D4 (tigm) IST10074B3 (tigm)
Private Clones OST337095 (lexicon) OST40106 (lexicon)
Severity of mutation (?) Insertion after 16% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000100993 (Chr11:21171707..21171741 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000100993 (Chr11:21171707..21171741 +)
Downstram Exon
ENSMUSE00000101000 (Chr11:21175001..21175132 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000486749 Chr11:21139284..21139374 No primer for this exon
upstream ENSMUSE00000101001 Chr11:21163202..21163357 No primer for this exon
upstream ENSMUSE00000720987 Chr11:21163202..21163357 No primer for this exon
upstream ENSMUSE00000101029 Chr11:21164633..21164874 No primer for this exon
upstream ENSMUSE00000680799 Chr11:21164669..21164874 No primer for this exon
upstream ENSMUSE00000100994 Chr11:21168788..21168866 No primer for this exon
upstream ENSMUSE00000100993 Chr11:21171707..21171741 No primer for this exon

*** Putative Vector Insertion (Chr 11: 21171742 - 21175000) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000101000 Chr11:21175001..21175132 No primer for this exon
downstream ENSMUSE00000101031 Chr11:21177678..21178063 No primer for this exon
downstream ENSMUSE00000101013 Chr11:21191036..21191162 No primer for this exon
downstream ENSMUSE00000100987 Chr11:21192027..21192134 No primer for this exon
downstream ENSMUSE00000101023 Chr11:21195344..21195399 No primer for this exon
downstream ENSMUSE00000101036 Chr11:21198757..21198853 No primer for this exon
downstream ENSMUSE00000101016 Chr11:21199981..21200321 No primer for this exon
downstream ENSMUSE00000101005 Chr11:21205654..21205783 No primer for this exon
downstream ENSMUSE00000100991 Chr11:21206369..21206550 No primer for this exon
downstream ENSMUSE00000100990 Chr11:21206912..21207024 No primer for this exon
downstream ENSMUSE00000100995 Chr11:21208742..21208805 No primer for this exon
downstream ENSMUSE00000100996 Chr11:21211080..21211185 No primer for this exon
downstream ENSMUSE00000100989 Chr11:21212243..21212330 No primer for this exon
downstream ENSMUSE00000461465 Chr11:21212854..21212975 No primer for this exon
downstream ENSMUSE00000101022 Chr11:21213087..21213167 No primer for this exon
downstream ENSMUSE00000101017 Chr11:21214922..21215029 No primer for this exon
downstream ENSMUSE00000101020 Chr11:21216795..21216889 No primer for this exon
downstream ENSMUSE00000383441 Chr11:21219756..21221136 No primer for this exon
downstream ENSMUSE00000655282 Chr11:21219756..21221125 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAGTTAATCGCCTTGCAGCAC Chr11:21171789..21171810 60.41 47.62 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTAGTCGTGACTGGGAAAACC Chr11:21174788..21174809 59.09 47.62 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020128