Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI2125
Trapped Gene
Arl10 (ENSMUSG00000025870)
Vector Insertion
Chr 13: 54677310 - 54680153
Public Clones AD0856 (sanger) CMHD-GT_336H5-3 (cmhd)
Private Clones not available
Severity of mutation (?) Insertion after 52% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000153728 (Chr13:54677111..54677309 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CCAAGAATTTCGAGGTGGAC Chr13:54677280..54677299 59.53 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000153728 (Chr13:54677111..54677309 +)
Downstram Exon
ENSMUSE00000153731 (Chr13:54680154..54680329 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CCAAGAATTTCGAGGTGGAC Chr13:54677280..54677299 59.53 50 CTTAGCCGGTCAGTCGAGTC Chr13:54680250..54680269 60.01 60

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000153730 Chr13:54676426..54676638 GCTGGGCTCTGTGCTCTTTA Chr13:54676512..54676531 60.68 55
upstream ENSMUSE00000153728 Chr13:54677111..54677309 CCAAGAATTTCGAGGTGGAC Chr13:54677280..54677299 59.53 50

*** Putative Vector Insertion (Chr 13: 54677310 - 54680153) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000153731 Chr13:54680154..54680329 CTTAGCCGGTCAGTCGAGTC Chr13:54680250..54680269 60.01 60
downstream ENSMUSE00000153729 Chr13:54682082..54682489 AACAACAATGCGGAAGAAGG Chr13:54682324..54682343 60.11 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCTGCCTACCAAGAATTTCG Chr13:54677273..54677293 59.85 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCTGCCTACCAAGAATTTCG Chr13:54677273..54677293 59.85 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000025870