Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21271
Trapped Gene
Dstn (ENSMUSG00000015932)
Vector Insertion
Chr 2: 143764429 - 143765687
Public Clones not available
Private Clones OST336601 (lexicon) OST277701 (lexicon) OST227189 (lexicon) OST208436 (lexicon)
OST188867 (lexicon)
Severity of mutation (?) Insertion after 62% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000169438 (Chr2:143764121..143764428 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000169438 (Chr2:143764121..143764428 +)
Downstram Exon
ENSMUSE00000557614 (Chr2:143765688..143765764 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000661484 Chr2:143741347..143741383 No primer for this exon
upstream ENSMUSE00000169438 Chr2:143764121..143764428 No primer for this exon

*** Putative Vector Insertion (Chr 2: 143764429 - 143765687) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000557614 Chr2:143765688..143765764 No primer for this exon
downstream ENSMUSE00000661483 Chr2:143767863..143769059 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCCGCTAGAGAACTGAGAGC Chr2:143764449..143764469 59.48 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAGCCAGTCTCCTACCGTGA Chr2:143764465..143764485 60.41 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000015932