Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21302
Trapped Gene
Spag7 (ENSMUSG00000018287)
Vector Insertion
Chr 11: 70478579 - 70478798
Public Clones not available
Private Clones OST335473 (lexicon) OST178033 (lexicon) OST165757 (lexicon) OST66214 (lexicon)
Severity of mutation (?) Insertion after 23% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000109641 (Chr11:70478799..70478866 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000109641 (Chr11:70478799..70478866 -)
Downstram Exon
ENSMUSE00000109648 (Chr11:70478490..70478578 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000383802 Chr11:70482699..70482795 No primer for this exon
upstream ENSMUSE00000109641 Chr11:70478799..70478866 No primer for this exon

*** Putative Vector Insertion (Chr 11: 70478579 - 70478798) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000109648 Chr11:70478490..70478578 No primer for this exon
downstream ENSMUSE00000109644 Chr11:70478285..70478369 No primer for this exon
downstream ENSMUSE00000109637 Chr11:70478080..70478169 No primer for this exon
downstream ENSMUSE00000109645 Chr11:70477822..70477978 No primer for this exon
downstream ENSMUSE00000374986 Chr11:70477292..70477722 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CAGCTTCTGGAAGGATTTGC Chr11:70478751..70478771 59.96 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TGCAGTACGTGACTGGGAAA Chr11:70478734..70478754 60.3 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000018287