Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21315
Trapped Gene
Dnajc17 (ENSMUSG00000034278)
Vector Insertion
Chr 2: 119009739 - 119011431
Public Clones not available
Private Clones OST334843 (lexicon) OST74060 (lexicon)
Severity of mutation (?) Insertion after 33% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000307739 (Chr2:119011432..119011519 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GGACCCAGAGACTTGACGAG Chr2:119011454..119011473 59.83 60 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000307739 (Chr2:119011432..119011519 -)
Downstram Exon
ENSMUSE00000307729 (Chr2:119009653..119009738 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GGACCCAGAGACTTGACGAG Chr2:119011454..119011473 59.83 60 GCTCCTGCTCTCCTCCTCTT Chr2:119009652..119009671 60.24 60

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000307758 Chr2:119034424..119034510 ACTAGAACCATGGCGGTGAC Chr2:119034491..119034510 60 55
upstream ENSMUSE00000307752 Chr2:119012082..119012151 AAGAAGGCGTACAGGCAAAA Chr2:119012129..119012148 59.88 45
upstream ENSMUSE00000307747 Chr2:119011765..119011823 AACTCTTCCACCAGCTGTCC Chr2:119011801..119011820 59.31 55
upstream ENSMUSE00000307739 Chr2:119011432..119011519 GGACCCAGAGACTTGACGAG Chr2:119011454..119011473 59.83 60

*** Putative Vector Insertion (Chr 2: 119009739 - 119011431) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000307729 Chr2:119009653..119009738 GCTCCTGCTCTCCTCCTCTT Chr2:119009652..119009671 60.24 60
downstream ENSMUSE00000307719 Chr2:119009335..119009431 ACTGACGGGAACCCTCTTCT Chr2:119009376..119009395 60.11 55
downstream ENSMUSE00000307708 Chr2:119006662..119006705 GGGTTCCTTTGCCTTCAGTA Chr2:119006651..119006670 59.17 50
downstream ENSMUSE00000307697 Chr2:119006223..119006300 CTCTGGAGTAGCCACCTTGG Chr2:119006227..119006246 59.86 60
downstream ENSMUSE00000307689 Chr2:119005621..119005701 CATTGCCTGCCTTCTTTCTG Chr2:119005631..119005650 60.9 50
downstream ENSMUSE00000307679 Chr2:119005088..119005198 CTCCAACCAGGAAACCTTCA Chr2:119005114..119005133 60.08 50
downstream ENSMUSE00000384836 Chr2:118998243..118998421 TCCCTTTCTGACAGCACTGA Chr2:118998377..118998396 59.54 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGACCCAGAGACTTGACGAG Chr2:119011452..119011472 59.83 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGACCCAGAGACTTGACGAG Chr2:119011452..119011472 59.83 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000034278