Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21327
Trapped Gene
Ppp2cb (ENSMUSG00000009630)
Vector Insertion
Chr 8: 34710491 - 34721126
Public Clones (sanger) 5SE167E02 (ggtc) IST14797A5 (tigm)
Private Clones OST334216 (lexicon) OST332585 (lexicon) OST330183 (lexicon) OST242397 (lexicon)
Severity of mutation (?) Insertion after 11% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000433955 (Chr8:34710097..34710490 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000433955 (Chr8:34710097..34710490 +)
Downstram Exon
ENSMUSE00000582550 (Chr8:34721127..34721336 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000433955 Chr8:34710097..34710490 No primer for this exon

*** Putative Vector Insertion (Chr 8: 34710491 - 34721126) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000582550 Chr8:34721127..34721336 No primer for this exon
downstream ENSMUSE00000582549 Chr8:34722220..34722393 No primer for this exon
downstream ENSMUSE00000210960 Chr8:34725922..34726011 No primer for this exon
downstream ENSMUSE00000210958 Chr8:34726138..34726299 No primer for this exon
downstream ENSMUSE00000582548 Chr8:34727499..34727617 No primer for this exon
downstream ENSMUSE00000410274 Chr8:34729594..34730266 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CATGCTCTATAATCGCCTTGC Chr8:34716533..34716554 59.85 47.62 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTGAGGAGGGGGAAAAACAG Chr8:34713477..34713497 61.34 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000009630