Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21399
Trapped Gene
Tle4 (ENSMUSG00000024642)
Vector Insertion
Chr 19: 14671345 - 14671857
Public Clones not available
Private Clones OST332204 (lexicon) OST215729 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000324705 (Chr19:14671858..14672473 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GTGGTGTTCGCAGAGTTTGA Chr19:14672435..14672454 59.88 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000324705 (Chr19:14671858..14672473 -)
Downstram Exon
ENSMUSE00000324695 (Chr19:14671247..14671344 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GTGGTGTTCGCAGAGTTTGA Chr19:14672435..14672454 59.88 50 TGTAAATTTGAAGGGTTGAGCA Chr19:14671287..14671308 59.62 36.36

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000324705 Chr19:14671858..14672473 GTGGTGTTCGCAGAGTTTGA Chr19:14672435..14672454 59.88 50

*** Putative Vector Insertion (Chr 19: 14671345 - 14671857) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000324695 Chr19:14671247..14671344 TGTAAATTTGAAGGGTTGAGCA Chr19:14671287..14671308 59.62 36.36
downstream ENSMUSE00000143959 Chr19:14670080..14670143 CCGCTGCATCTCTGTCTTCT Chr19:14670070..14670089 60.7 55
downstream ENSMUSE00000547755 Chr19:14668874..14668918 GTTCAACCCATAGGACATTTCA Chr19:14668870..14668891 58.81 40.91
downstream ENSMUSE00000143948 Chr19:14638879..14638941 GGGACAGGAAAGGAATGACC Chr19:14638862..14638881 60.69 55
downstream ENSMUSE00000547744 Chr19:14619278..14619352 No primer for this exon
downstream ENSMUSE00000642704 Chr19:14592262..14592463 TGCTGAGCTTGGAGTTGTTG Chr19:14592422..14592441 60.17 50
downstream ENSMUSE00000693631 Chr19:14590849..14590865 No primer for this exon
downstream ENSMUSE00000547675 Chr19:14542642..14542761 GAAACTGGCTGATGGGGATA Chr19:14542710..14542729 59.89 50
downstream ENSMUSE00000547731 Chr19:14541670..14541723 No primer for this exon
downstream ENSMUSE00000143962 Chr19:14541039..14541191 GTCTTGTCCAGGCCATTCTC Chr19:14541108..14541127 59.66 55
downstream ENSMUSE00000143949 Chr19:14539776..14539908 GGGTAGGTGCATCAGTTCGT Chr19:14539820..14539839 60 55
downstream ENSMUSE00000143957 Chr19:14539234..14539427 CTGGTCAGCTCTCCGTTCAT Chr19:14539312..14539331 60.41 55
downstream ENSMUSE00000143943 Chr19:14538799..14538875 CCTCCTGGAATGCCTGTTAG Chr19:14538783..14538802 59.69 55
downstream ENSMUSE00000371594 Chr19:14529231..14529480 GACCTTAACACAGCCCTTGC Chr19:14529263..14529282 59.74 55
downstream ENSMUSE00000693616 Chr19:14528197..14528207 No primer for this exon
downstream ENSMUSE00000547711 Chr19:14528069..14528316 ATTAAGGTGCGACCATCAGG Chr19:14528239..14528258 59.96 50
downstream ENSMUSE00000693615 Chr19:14528069..14528116 GATCCCACACTGCGATGTTA Chr19:14528069..14528088 59.53 50
downstream ENSMUSE00000143947 Chr19:14526910..14527057 TGTTGTCCAAACCACCTGTC Chr19:14526950..14526969 59.42 50
downstream ENSMUSE00000547696 Chr19:14526554..14526704 GGCAATAGCCCAATGAAAAG Chr19:14526661..14526680 59.55 45
downstream ENSMUSE00000547693 Chr19:14526133..14526209 GAATATGCTGGCCCCATAAG Chr19:14526114..14526133 59.39 50
downstream ENSMUSE00000706838 Chr19:14522640..14524331 GAGGGGTGGAGCATCAAATA Chr19:14523824..14523843 59.89 50
downstream ENSMUSE00000412382 Chr19:14522562..14524331 GAGGGGTGGAGCATCAAATA Chr19:14523824..14523843 59.89 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CGACCTGAGCAAGATGTACC Chr19:14671873..14671893 58.31 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CGACCTGAGCAAGATGTACC Chr19:14671873..14671893 58.31 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024642