Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21439
Trapped Gene
Magmas (ENSMUSG00000014301)
Vector Insertion
Chr 16: 4617263 - 4617918
Public Clones not available
Private Clones OST330043 (lexicon) OST165895 (lexicon)
Severity of mutation (?) Insertion after 24% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000414559 (Chr16:4617919..4618003 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000414559 (Chr16:4617919..4618003 -)
Downstram Exon
ENSMUSE00000470644 (Chr16:4617126..4617262 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000704393 Chr16:4624851..4624936 No primer for this exon
upstream ENSMUSE00000414559 Chr16:4617919..4618003 No primer for this exon

*** Putative Vector Insertion (Chr 16: 4617263 - 4617918) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000470644 Chr16:4617126..4617262 No primer for this exon
downstream ENSMUSE00000465823 Chr16:4616813..4616878 No primer for this exon
downstream ENSMUSE00000294384 Chr16:4616474..4616633 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGCAGGAGTTTGCAGGTAAG Chr16:4617912..4617932 59.88 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGCAGGAGTTTGCAGGTAAG Chr16:4617912..4617932 59.88 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000014301