Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21455
Trapped Gene
Uhrf2 (ENSMUSG00000024817)
Vector Insertion
Chr 19: 30105422 - 30113553
Public Clones not available
Private Clones OST329816 (lexicon) OST329809 (lexicon)
Severity of mutation (?) Insertion after 6% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000346551 (Chr19:30105003..30105421 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CGGTCTAAGAGCGTCAGTCC Chr19:30105104..30105123 60.01 60 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000346551 (Chr19:30105003..30105421 +)
Downstram Exon
ENSMUSE00000145220 (Chr19:30113554..30113787 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CGGTCTAAGAGCGTCAGTCC Chr19:30105104..30105123 60.01 60 TGGACGAACTAGCAGCTGAA Chr19:30113628..30113647 59.74 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000346551 Chr19:30105003..30105421 CGGTCTAAGAGCGTCAGTCC Chr19:30105104..30105123 60.01 60
upstream ENSMUSE00000692227 Chr19:30105079..30105379 CGGTCTAAGAGCGTCAGTCC Chr19:30105104..30105123 60.01 60

*** Putative Vector Insertion (Chr 19: 30105422 - 30113553) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000145220 Chr19:30113554..30113787 TGGACGAACTAGCAGCTGAA Chr19:30113628..30113647 59.74 50
downstream ENSMUSE00000255509 Chr19:30130725..30130984 GCTTCAAACCAAGCACCAAG Chr19:30130780..30130799 60.81 50
downstream ENSMUSE00000692225 Chr19:30130725..30131653 TGCGCTCTTAAAGCCAAGAT Chr19:30131511..30131530 60.12 45
downstream ENSMUSE00000145216 Chr19:30145733..30145951 CGTGGTCGAAGATCCTTAACA Chr19:30145785..30145805 60.12 47.62
downstream ENSMUSE00000255475 Chr19:30148384..30148493 TCGGCAGTCATTTAATGTGC Chr19:30148417..30148436 59.69 45
downstream ENSMUSE00000255426 Chr19:30149574..30149760 CCACACAGGTCACATTCAGG Chr19:30149607..30149626 60 55
downstream ENSMUSE00000145204 Chr19:30152504..30152627 TCCAGCCTTTACCACCTCAC Chr19:30152555..30152574 60.11 55
downstream ENSMUSE00000145234 Chr19:30153030..30153137 No primer for this exon
downstream ENSMUSE00000145247 Chr19:30154329..30154433 TCCAGCCAGGACAAGAGAAT Chr19:30154418..30154437 59.8 50
downstream ENSMUSE00000145212 Chr19:30157224..30157330 TAGTGTCTGGTCAGCCGATG Chr19:30157319..30157338 59.85 55
downstream ENSMUSE00000145238 Chr19:30160736..30160898 AATTCCGAGACTCTGCTCCA Chr19:30160800..30160819 59.95 50
downstream ENSMUSE00000145227 Chr19:30161666..30161806 CTCCTTGATCGTTCGATTCC Chr19:30161790..30161809 59.63 50
downstream ENSMUSE00000145200 Chr19:30162969..30163065 TTCCTTTTCCGAGGGGTAAC Chr19:30163001..30163020 60.29 50
downstream ENSMUSE00000145246 Chr19:30163676..30163833 ATGCCTTCAGCACTTTGGAG Chr19:30163714..30163733 60.4 50
downstream ENSMUSE00000145208 Chr19:30166531..30166629 GTTCTTGGCAGCACACACAC Chr19:30166585..30166604 60.37 55
downstream ENSMUSE00000545520 Chr19:30167208..30168210 AGACATGGTCCAGCTCTGCT Chr19:30167448..30167467 60.02 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGTGCCAGCGCCTGTTCTAC Chr19:30108391..30108411 62.84 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGTGCCAGCGCCTGTTCTAC Chr19:30108391..30108411 62.84 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024817