Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21726
Trapped Gene
E130303B06Rik (ENSMUSG00000013155)
Vector Insertion
Chr 8: 108230639 - 108231448
Public Clones not available
Private Clones OST319697 (lexicon)
Severity of mutation (?) Insertion after 27% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000214416 (Chr8:108231449..108231643 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000214416 (Chr8:108231449..108231643 -)
Downstram Exon
ENSMUSE00000214412 (Chr8:108230466..108230638 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000381930 Chr8:108231724..108232090 No primer for this exon
upstream ENSMUSE00000214416 Chr8:108231449..108231643 No primer for this exon

*** Putative Vector Insertion (Chr 8: 108230639 - 108231448) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000214412 Chr8:108230466..108230638 No primer for this exon
downstream ENSMUSE00000214411 Chr8:108228535..108228660 No primer for this exon
downstream ENSMUSE00000214415 Chr8:108228251..108228414 No primer for this exon
downstream ENSMUSE00000214410 Chr8:108228013..108228149 No primer for this exon
downstream ENSMUSE00000377274 Chr8:108227553..108227921 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACGGGGTATTGAGGCATTCT Chr8:108231413..108231433 60.71 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACGGGGTATTGAGGCATTCT Chr8:108231413..108231433 60.71 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000013155