Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21730
Trapped Gene
Zfp330 (ENSMUSG00000031711)
Vector Insertion
Chr 8: 85293299 - 85294699
Public Clones not available
Private Clones OST319534 (lexicon)
Severity of mutation (?) Insertion after 22% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000212288 (Chr8:85294700..85294770 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GCGGCAGAAGAATAGAGCAT Chr8:85294751..85294770 59.58 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000212288 (Chr8:85294700..85294770 -)
Downstram Exon
ENSMUSE00000212283 (Chr8:85293219..85293298 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GCGGCAGAAGAATAGAGCAT Chr8:85294751..85294770 59.58 50 CACACCAGCATGCTTGATAA Chr8:85293221..85293240 58.29 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000581568 Chr8:85297956..85298059 CTGCTTCTGCCGTTCTAGCC Chr8:85297994..85298013 62.48 60
upstream ENSMUSE00000400309 Chr8:85296779..85296910 GAAACAACTGCGAGCATCAA Chr8:85296823..85296842 59.99 45
upstream ENSMUSE00000212286 Chr8:85295238..85295257 No primer for this exon
upstream ENSMUSE00000212288 Chr8:85294700..85294770 GCGGCAGAAGAATAGAGCAT Chr8:85294751..85294770 59.58 50

*** Putative Vector Insertion (Chr 8: 85293299 - 85294699) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000212283 Chr8:85293219..85293298 CACACCAGCATGCTTGATAA Chr8:85293221..85293240 58.29 45
downstream ENSMUSE00000212284 Chr8:85291112..85291238 CTCGCTCACATTCCACACAC Chr8:85291105..85291124 60.32 55
downstream ENSMUSE00000212287 Chr8:85290568..85290672 GGCAAAACGAACAGCTGAAT Chr8:85290621..85290640 60.26 45
downstream ENSMUSE00000212292 Chr8:85289911..85289957 CAAGTCGATTGCAGGAAACA Chr8:85289915..85289934 59.84 45
downstream ENSMUSE00000212291 Chr8:85288754..85288871 GGCAAGGAGGTTGTTTTCCT Chr8:85288780..85288799 60.48 50
downstream ENSMUSE00000541833 Chr8:85288163..85288390 ACTTGTCCGACGACAGGTTC Chr8:85288276..85288295 60.16 55
downstream ENSMUSE00000681789 Chr8:85287991..85288010 No primer for this exon
downstream ENSMUSE00000412168 Chr8:85287520..85288390 CAACCGCTAATGCAGAGTGA Chr8:85287660..85287679 60.01 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTTTGTGTGTAATCGCCTTGC Chr8:85294636..85294657 60.56 47.62 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATCAAATGTTTGTGTGCGTGA Chr8:85294643..85294664 60.03 38.1 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031711