Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21752
Trapped Gene
Gin1 (ENSMUSG00000026333)
Vector Insertion
Chr 1: 99666833 - 99672023
Public Clones (sanger)
Private Clones OST318972 (lexicon) OST281289 (lexicon) OST280537 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000706938 (Chr1:99666745..99666832 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AACCTGCCGTTTGCACTTAG Chr1:99666803..99666822 60.31 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000706938 (Chr1:99666745..99666832 +)
Downstram Exon
ENSMUSE00000709792 (Chr1:99672024..99672169 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AACCTGCCGTTTGCACTTAG Chr1:99666803..99666822 60.31 50 GATATTCCCCTGTGCGCTTA Chr1:99672106..99672125 60.06 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000706938 Chr1:99666745..99666832 AACCTGCCGTTTGCACTTAG Chr1:99666803..99666822 60.31 50
upstream ENSMUSE00000693398 Chr1:99666756..99666832 AACCTGCCGTTTGCACTTAG Chr1:99666803..99666822 60.31 50
upstream ENSMUSE00000597881 Chr1:99666774..99666832 AACCTGCCGTTTGCACTTAG Chr1:99666803..99666822 60.31 50

*** Putative Vector Insertion (Chr 1: 99666833 - 99672023) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000158134 Chr1:99672024..99672169 GATATTCCCCTGTGCGCTTA Chr1:99672106..99672125 60.06 50
downstream ENSMUSE00000709792 Chr1:99672024..99672169 GATATTCCCCTGTGCGCTTA Chr1:99672106..99672125 60.06 50
downstream ENSMUSE00000158130 Chr1:99673903..99674096 ACGGACGTCCAGTAGTAGCC Chr1:99674077..99674096 59.24 60
downstream ENSMUSE00000693388 Chr1:99673903..99674716 AGCCATACCCACTGCTTGAC Chr1:99674107..99674126 60.14 55
downstream ENSMUSE00000693397 Chr1:99679504..99679809 GGAAAGGTCCCATCAGATCA Chr1:99679627..99679646 59.86 50
downstream ENSMUSE00000659722 Chr1:99679771..99679809 TGAATTCATCTCTTTGGTCCATT Chr1:99679801..99679823 59.83 34.78
downstream ENSMUSE00000158132 Chr1:99681330..99681521 CACTTCCAGAAGCACGAGAA Chr1:99681399..99681418 59.16 50
downstream ENSMUSE00000283355 Chr1:99681632..99681799 CGCCATCAGCCTCTCTAATC Chr1:99681770..99681789 59.94 55
downstream ENSMUSE00000158135 Chr1:99682553..99682835 GGCCTAAGGTGGGACATTTT Chr1:99682812..99682831 60.19 50
downstream ENSMUSE00000158129 Chr1:99688874..99690286 CGTGATCTGCCACTATCGAA Chr1:99688918..99688937 59.82 50
downstream ENSMUSE00000693395 Chr1:99688874..99689342 GTCGTGATCTGCCACTATCG Chr1:99688920..99688939 59.27 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AACCTGCCGTTTGCACTTAG Chr1:99666804..99666824 60.31 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AACCTGCCGTTTGCACTTAG Chr1:99666804..99666824 60.31 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000026333