Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI21758
Trapped Gene
Csrp2 (ENSMUSG00000020186)
Vector Insertion
Chr 10: 110372373 - 110374792
Public Clones not available
Private Clones OST318770 (lexicon) OST209211 (lexicon) OST197823 (lexicon) OST181442 (lexicon)
OST122299 (lexicon) OST31039 (lexicon)
Severity of mutation (?) Insertion after 48% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000101593 (Chr10:110372204..110372372 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000101593 (Chr10:110372204..110372372 +)
Downstram Exon
ENSMUSE00000101596 (Chr10:110374793..110374922 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000369473 Chr10:110357235..110357310 No primer for this exon
upstream ENSMUSE00000101586 Chr10:110369011..110369123 No primer for this exon
upstream ENSMUSE00000101593 Chr10:110372204..110372372 No primer for this exon

*** Putative Vector Insertion (Chr 10: 110372373 - 110374792) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000101596 Chr10:110374793..110374922 No primer for this exon
downstream ENSMUSE00000573921 Chr10:110375693..110375786 No primer for this exon
downstream ENSMUSE00000438184 Chr10:110376267..110376678 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGAACAGCTCCTGTCGCTCT Chr10:110372405..110372425 59.35 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCAAGCCAGAGAGGTGAGAGA Chr10:110372361..110372382 60.27 52.38 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020186