Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI22098
Trapped Gene
D19Wsu162e (ENSMUSG00000047731)
Vector Insertion
Chr 19: 46698070 - 46718842
Public Clones not available
Private Clones OST308664 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000689283 (Chr19:46698043..46698069 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CTTTCCTCTGGGGGCTTAGA Chr19:46698047..46698066 60.69 55 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000689283 (Chr19:46698043..46698069 +)
Downstram Exon
ENSMUSE00000471790 (Chr19:46718843..46718945 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CTTTCCTCTGGGGGCTTAGA Chr19:46698047..46698066 60.69 55 GCAGCACTGAGACTGTCCAC Chr19:46718926..46718945 59.61 60

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000708213 Chr19:46673627..46673783 GGGAAGAAGAGAGGGAGGAG Chr19:46673630..46673649 59.37 60
upstream ENSMUSE00000713184 Chr19:46673627..46673783 GGGAAGAAGAGAGGGAGGAG Chr19:46673630..46673649 59.37 60
upstream ENSMUSE00000641158 Chr19:46681202..46681249 GTGCGCTTGAGAAGTCAGAA Chr19:46681202..46681221 59.32 50
upstream ENSMUSE00000689283 Chr19:46698043..46698069 CTTTCCTCTGGGGGCTTAGA Chr19:46698047..46698066 60.69 55

*** Putative Vector Insertion (Chr 19: 46698070 - 46718842) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000471790 Chr19:46718843..46718945 GCAGCACTGAGACTGTCCAC Chr19:46718926..46718945 59.61 60
downstream ENSMUSE00000706656 Chr19:46718843..46719057 CCCCTGGACAAGGACTTACA Chr19:46718967..46718986 59.96 55
downstream ENSMUSE00000472696 Chr19:46726964..46727125 AAACGGCAACGCTGAGTAGT Chr19:46727121..46727140 59.94 50
downstream ENSMUSE00000389933 Chr19:46728455..46731875 CTGCAAATCTGACCGTAGCA Chr19:46731686..46731705 60.01 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGTTTGAGGAACTGGGCTAA Chr19:46710055..46710075 59.17 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGTTTGAGGAACTGGGCTAA Chr19:46710055..46710075 59.17 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000047731