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About
UniTrap UNI22132
Trapped Gene
Syn1 (
ENSMUSG00000037217
)
Vector Insertion
Chr X: 20486555 - 20486650
Public Clones
not available
Private Clones
OST307399 (lexicon)
Severity of mutation
(?)
Insertion after 22% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000324391 (ChrX:20486651..20486708 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
GGGAAGAAGATCCATGGAGAA
ChrX:20486672..20486692
60.4
47.62
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000324391 (ChrX:20486651..20486708 -)
Downstram Exon
ENSMUSE00000324386 (ChrX:20486463..20486554 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
GGGAAGAAGATCCATGGAGAA
ChrX:20486672..20486692
60.4
47.62
TCCGAAGAACTTCCATGTCC
ChrX:20486460..20486479
60.05
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000468803
ChrX:20497539..20498022
GCTTCTTCTCGTCGCTGTCT
ChrX:20497673..20497692
59.9
55
upstream
ENSMUSE00000702543
ChrX:20497539..20498130
GCTTCTTCTCGTCGCTGTCT
ChrX:20497673..20497692
59.9
55
upstream
ENSMUSE00000324391
ChrX:20486651..20486708
GGGAAGAAGATCCATGGAGAA
ChrX:20486672..20486692
60.4
47.62
*** Putative Vector Insertion (Chr X: 20486555 - 20486650) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000324386
ChrX:20486463..20486554
TCCGAAGAACTTCCATGTCC
ChrX:20486460..20486479
60.05
50
downstream
ENSMUSE00000624562
ChrX:20485077..20485233
CATTACGTGCCATGCTGAAG
ChrX:20485153..20485172
60.28
50
downstream
ENSMUSE00000378197
ChrX:20484203..20484292
GAGGGAATTCCTCTGTTCCA
ChrX:20484219..20484238
59.06
50
downstream
ENSMUSE00000324360
ChrX:20442972..20443034
CCACAGGGTATGTTGTGCTG
ChrX:20442988..20443007
60.03
55
downstream
ENSMUSE00000624561
ChrX:20442245..20442387
GTTCTGCCCAATCTTCTGGA
ChrX:20442237..20442256
60.19
50
downstream
ENSMUSE00000324341
ChrX:20442081..20442155
GGTCTTCCAGTTACCCGACA
ChrX:20442103..20442122
59.97
55
downstream
ENSMUSE00000324329
ChrX:20441887..20441989
GATGTCAAGTCCCCCAAAAA
ChrX:20441916..20441935
59.77
45
downstream
ENSMUSE00000324320
ChrX:20440710..20440856
AGTTCCACGATGAGCTGCTT
ChrX:20440767..20440786
60.02
50
downstream
ENSMUSE00000324312
ChrX:20440309..20440396
No primer for this exon
downstream
ENSMUSE00000495548
ChrX:20439899..20440216
AAAGATGTTGCTGGCCTTGT
ChrX:20440108..20440127
59.74
45
downstream
ENSMUSE00000556820
ChrX:20439625..20439814
No primer for this exon
downstream
ENSMUSE00000624560
ChrX:20439625..20440216
AAAGATGTTGCTGGCCTTGT
ChrX:20440108..20440127
59.74
45
downstream
ENSMUSE00000702557
ChrX:20437641..20438735
GGGCTGTCTAGGGGTTTAGG
ChrX:20438540..20438559
59.96
60
downstream
ENSMUSE00000702539
ChrX:20437637..20438697
GGGCTGTCTAGGGGTTTAGG
ChrX:20438540..20438559
59.96
60
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
ACCAATTGTAATCGCCTTGC
ChrX:20486587..20486607
59.97
45
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
Come back to gene
ENSMUSG00000037217