Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI22188
Trapped Gene
Rpl18 (ENSMUSG00000059070)
Vector Insertion
Chr 7: 52975858 - 52976083
Public Clones not available
Private Clones OST303879 (lexicon)
Severity of mutation (?) Insertion after 87% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000500647 (Chr7:52975664..52975857 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GTACCGACATTTTGGCAAGG Chr7:52975810..52975829 60.37 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000500647 (Chr7:52975664..52975857 +)
Downstram Exon
ENSMUSE00000513613 (Chr7:52976084..52976200 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GTACCGACATTTTGGCAAGG Chr7:52975810..52975829 60.37 50 No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000674277 Chr7:52970827..52971212 GGGAGGCTCATGGACTTACA Chr7:52971100..52971119 60.07 55
upstream ENSMUSE00000674276 Chr7:52971518..52973509 GTTTACTCGGTCCTCCCACA Chr7:52973294..52973313 59.97 55
upstream ENSMUSE00000674278 Chr7:52973474..52973509 No primer for this exon
upstream ENSMUSE00000517867 Chr7:52974623..52974709 CACAACAAGGACCGAAAGGT Chr7:52974638..52974657 60.01 50
upstream ENSMUSE00000520562 Chr7:52974899..52975006 CACCTTCAATCAGGTTGTGC Chr7:52974931..52974950 59.14 50
upstream ENSMUSE00000515956 Chr7:52975290..52975388 CACAGATGATGTGCGGATTC Chr7:52975349..52975368 60.08 50
upstream ENSMUSE00000500647 Chr7:52975664..52975857 GTACCGACATTTTGGCAAGG Chr7:52975810..52975829 60.37 50

*** Putative Vector Insertion (Chr 7: 52975858 - 52976083) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000512079 Chr7:52976084..52976204 No primer for this exon
downstream ENSMUSE00000513613 Chr7:52976084..52976200 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GTACCGACATTTTGGCAAGG Chr7:52975811..52975831 60.37 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GTACCGACATTTTGGCAAGG Chr7:52975811..52975831 60.37 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000059070