Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI22275
Trapped Gene
Gm1631 (ENSMUSG00000064263)
Vector Insertion
Chr 2: 71568765 - 71568956
Public Clones not available
Private Clones OST301819 (lexicon) OST190492 (lexicon) OST127209 (lexicon) OST68517 (lexicon)
Severity of mutation (?) Insertion after 66% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000690500 (Chr2:71568766..71569023 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GCCAAATTGCCATCTGTTTC Chr2:71568898..71568917 60.46 45 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000690500 (Chr2:71568766..71569023 +)
Downstram Exon
ENSMUSE00000690503 (Chr2:71568767..71568955 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GCCAAATTGCCATCTGTTTC Chr2:71568898..71568917 60.46 45 TTGCATATTCCACTCGCTTG Chr2:71568854..71568873 59.83 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000690502 Chr2:71557446..71557565 AGTGCATGGAGAGGAACCAG Chr2:71557533..71557552 60.26 55
upstream ENSMUSE00000492955 Chr2:71557474..71557565 AGTGCATGGAGAGGAACCAG Chr2:71557533..71557552 60.26 55
upstream ENSMUSE00000503171 Chr2:71561347..71561553 CAAGCTCAAGCAGCAATCAG Chr2:71561353..71561372 59.89 50
upstream ENSMUSE00000506080 Chr2:71568407..71568490 TGTTCTAGCCTGGTGTTGGA Chr2:71568452..71568471 59.29 50
upstream ENSMUSE00000690501 Chr2:71568407..71568489 ACCCTACAACCCAGGGAAAT Chr2:71568425..71568444 59.55 50

*** Putative Vector Insertion (Chr 2: 71568765 - 71568956) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000690500 Chr2:71568766..71569023 TTGCATATTCCACTCGCTTG Chr2:71568854..71568873 59.83 45
downstream ENSMUSE00000690503 Chr2:71568767..71568955 TTGCATATTCCACTCGCTTG Chr2:71568854..71568873 59.83 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTCTAATCGCCTTGCAGCAC Chr2:71568813..71568833 61.07 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AATCTCCGTGACTGGGAAAA Chr2:71568810..71568830 59.53 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000064263