Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI2232
Trapped Gene
Plk1 (ENSMUSG00000030867)
Vector Insertion
Chr 7: 129303465 - 129304049
Public Clones AS0407 (sanger)
Private Clones not available
Severity of mutation (?) Insertion after 22% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000395577 (Chr7:129302999..129303464 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ACGTCGTAGGCTTCCATGAC Chr7:129303391..129303410 60.14 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000395577 (Chr7:129302999..129303464 +)
Downstram Exon
ENSMUSE00000204485 (Chr7:129304050..129304218 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ACGTCGTAGGCTTCCATGAC Chr7:129303391..129303410 60.14 55 CTCGTTCAGGAAGAGGTTGC Chr7:129304202..129304221 59.99 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000395577 Chr7:129302999..129303464 ACGTCGTAGGCTTCCATGAC Chr7:129303391..129303410 60.14 55

*** Putative Vector Insertion (Chr 7: 129303465 - 129304049) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000204485 Chr7:129304050..129304218 CTCGTTCAGGAAGAGGTTGC Chr7:129304202..129304221 59.99 55
downstream ENSMUSE00000204489 Chr7:129305021..129305165 ATTCCACTTTGGTTGCCAAG Chr7:129305053..129305072 59.97 45
downstream ENSMUSE00000258376 Chr7:129305907..129306000 TAGGCACGAGGTCTCAAAGG Chr7:129305955..129305974 60.39 55
downstream ENSMUSE00000204494 Chr7:129307534..129307753 TGGTGAGGCAGGTAATAGGG Chr7:129307681..129307700 59.95 55
downstream ENSMUSE00000204486 Chr7:129311111..129311266 GACCACCGGTTCCTCTTTCT Chr7:129311166..129311185 60.49 55
downstream ENSMUSE00000204481 Chr7:129312080..129312157 CCGAATAGTCCACCCACTTG Chr7:129312145..129312164 60.37 55
downstream ENSMUSE00000204492 Chr7:129312343..129312497 ATAGGACTCCGTGCCATCAC Chr7:129312461..129312480 59.96 55
downstream ENSMUSE00000478737 Chr7:129312595..129312777 AACCACGTTCGTAGGTAGGG Chr7:129312716..129312735 58.98 55
downstream ENSMUSE00000411403 Chr7:129312901..129313387 CACCAGTCCGAAGGAGAGAG Chr7:129313129..129313148 59.98 60

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCAGGAGGGTGAGTGTTTCA Chr7:129303458..129303478 61.26 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAACTTGCTGACGTGACTGG Chr7:129303505..129303525 59.47 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000030867