Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI22336
Trapped Gene
Oaz2 (ENSMUSG00000040652)
Vector Insertion
Chr 9: 65534161 - 65535604
Public Clones IST14475A1 (tigm)
Private Clones OST299889 (lexicon) OST274638 (lexicon) OST221690 (lexicon) OST196863 (lexicon)
OST193774 (lexicon)
Severity of mutation (?) Insertion after 31% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000346280 (Chr9:65534080..65534160 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CCTCACCCACTGTCGAAGAT Chr9:65534086..65534105 60.11 55 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000346280 (Chr9:65534080..65534160 +)
Downstram Exon
ENSMUSE00000246250 (Chr9:65535605..65535780 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CCTCACCCACTGTCGAAGAT Chr9:65534086..65534105 60.11 55 GGAAGTGGACGATGTGAGGT Chr9:65535674..65535693 59.97 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000363133 Chr9:65524397..65524572 GAGCCACCGAGGATGATAAA Chr9:65524541..65524560 60.04 50
upstream ENSMUSE00000409150 Chr9:65534003..65534078 AGTAAGTGTCCCCAGCTCCA Chr9:65534019..65534038 59.72 55
upstream ENSMUSE00000346280 Chr9:65534080..65534160 CCTCACCCACTGTCGAAGAT Chr9:65534086..65534105 60.11 55

*** Putative Vector Insertion (Chr 9: 65534161 - 65535604) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000246250 Chr9:65535605..65535780 GGAAGTGGACGATGTGAGGT Chr9:65535674..65535693 59.97 55
downstream ENSMUSE00000246230 Chr9:65536628..65536713 CCCTTCCTGAAGCAGATGAA Chr9:65536702..65536721 60.33 50
downstream ENSMUSE00000246214 Chr9:65536902..65538104 AGGCAAGAGCTCTGGTGGTA Chr9:65537775..65537794 60.01 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGCAGGGATCCTTCTCTCTC Chr9:65534126..65534146 60.3 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGCAGGGATCCTTCTCTCTC Chr9:65534126..65534146 60.3 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000040652