Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI22473
Trapped Gene
Psmb1 (ENSMUSG00000014769)
Vector Insertion
Chr 17: 15630144 - 15631379
Public Clones not available
Private Clones OST296127 (lexicon) OST195458 (lexicon) OST48315 (lexicon)
Severity of mutation (?) Insertion after 30% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000135935 (Chr17:15631380..15631487 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000135935 (Chr17:15631380..15631487 -)
Downstram Exon
ENSMUSE00000135936 (Chr17:15630062..15630143 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000135934 Chr17:15635117..15635262 No primer for this exon
upstream ENSMUSE00000135935 Chr17:15631380..15631487 No primer for this exon

*** Putative Vector Insertion (Chr 17: 15630144 - 15631379) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000135936 Chr17:15630062..15630143 No primer for this exon
downstream ENSMUSE00000135932 Chr17:15627120..15627249 No primer for this exon
downstream ENSMUSE00000135931 Chr17:15614279..15614385 No primer for this exon
downstream ENSMUSE00000454699 Chr17:15612924..15613169 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CGAGATAGCCCCAAATGCTA Chr17:15631384..15631404 60.19 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CGAGATAGCCCCAAATGCTA Chr17:15631384..15631404 60.19 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000014769