Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI22513
Trapped Gene
Tfpi (ENSMUSG00000027082)
Vector Insertion
Chr 2: 84294155 - 84298179
Public Clones not available
Private Clones OST294712 (lexicon) OST244215 (lexicon) OST206509 (lexicon) OST67045 (lexicon)
Severity of mutation (?) Insertion after 12% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000712250 (Chr2:84298180..84298290 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AGCCTTGTTCCCGAGTTTCT Chr2:84298212..84298231 60.25 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000712250 (Chr2:84298180..84298290 -)
Downstram Exon
ENSMUSE00000165893 (Chr2:84293957..84294154 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AGCCTTGTTCCCGAGTTTCT Chr2:84298212..84298231 60.25 50 TTCTCGTTCCCTTCACATCC Chr2:84293979..84293998 60.05 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000688739 Chr2:84316898..84316932 No primer for this exon
upstream ENSMUSE00000688736 Chr2:84316848..84316905 AAAGTCCTTCCTCGCTGTGA Chr2:84316869..84316888 59.99 50
upstream ENSMUSE00000688747 Chr2:84316848..84316920 AAAGTCCTTCCTCGCTGTGA Chr2:84316869..84316888 59.99 50
upstream ENSMUSE00000688756 Chr2:84314919..84315437 TTTCTCAGCAGGAAGCCTGT Chr2:84315074..84315093 60.13 50
upstream ENSMUSE00000415207 Chr2:84314100..84314192 CCCGAGCAACCTTTACAGAG Chr2:84314126..84314145 59.87 55
upstream ENSMUSE00000688753 Chr2:84314100..84314342 CCCGAGCAACCTTTACAGAG Chr2:84314126..84314145 59.87 55
upstream ENSMUSE00000237244 Chr2:84313856..84313956 GGACCTGTCTCCGGATTTCT Chr2:84313922..84313941 60.46 55
upstream ENSMUSE00000600985 Chr2:84313856..84313941 GGACCTGTCTCCGGATTTCT Chr2:84313922..84313941 60.46 55
upstream ENSMUSE00000237237 Chr2:84298180..84298290 AGCCTTGTTCCCGAGTTTCT Chr2:84298212..84298231 60.25 50
upstream ENSMUSE00000712250 Chr2:84298180..84298290 AGCCTTGTTCCCGAGTTTCT Chr2:84298212..84298231 60.25 50

*** Putative Vector Insertion (Chr 2: 84294155 - 84298179) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000165893 Chr2:84293957..84294154 TTCTCGTTCCCTTCACATCC Chr2:84293979..84293998 60.05 50
downstream ENSMUSE00000165888 Chr2:84292714..84292752 GCTGCCTTCACAGCTGTCTT Chr2:84292703..84292722 60.74 55
downstream ENSMUSE00000237215 Chr2:84284897..84285073 CACGAATCGTTCACACTGCT Chr2:84284948..84284967 59.9 50
downstream ENSMUSE00000165889 Chr2:84284167..84284295 TGGGGAACCACGATGTTATT Chr2:84284171..84284190 60.05 45
downstream ENSMUSE00000688761 Chr2:84284167..84284277 TGGGGAACCACGATGTTATT Chr2:84284171..84284190 60.05 45
downstream ENSMUSE00000688738 Chr2:84283360..84283469 ACGGAACTCAGAAAGCCTTG Chr2:84283378..84283397 59.47 50
downstream ENSMUSE00000688748 Chr2:84283027..84283082 No primer for this exon
downstream ENSMUSE00000565912 Chr2:84280297..84283082 AACCCATCGGTGATGACATT Chr2:84282676..84282695 60.06 45
downstream ENSMUSE00000688735 Chr2:84280297..84283469 AACCCATCGGTGATGACATT Chr2:84282676..84282695 60.06 45
downstream ENSMUSE00000165890 Chr2:84274399..84274578 TCCCCCACATCCAGTGTAGT Chr2:84274429..84274448 60.24 55
downstream ENSMUSE00000377228 Chr2:84273014..84273341 TGGTCACCAATGGAAACAAG Chr2:84273114..84273133 59.39 45
downstream ENSMUSE00000688760 Chr2:84273012..84273341 TGGTCACCAATGGAAACAAG Chr2:84273114..84273133 59.39 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTCTCATAATCGCCTTGCAG Chr2:84298114..84298134 59.02 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCTCACGTGACTGGGAAAAC Chr2:84298113..84298133 58.7 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000027082