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UniTrap UNI22525
Trapped Gene
Rasgrp2 (ENSMUSG00000032946)
Vector Insertion
Chr 19: 6401701 - 6401864
Public Clones not available
Private Clones OST293127 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000695736 (Chr19:6401702..6401863 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ATGACGAGCACTCTGGACCT Chr19:6401791..6401810 59.87 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000695736 (Chr19:6401702..6401863 +)
Downstram Exon
ENSMUSE00000413610 (Chr19:6401716..6401863 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ATGACGAGCACTCTGGACCT Chr19:6401791..6401810 59.87 55 AGGTCCAGAGTGCTCGTCAT Chr19:6401813..6401832 59.87 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000695762 Chr19:6399340..6399517 AAACCAGCTCGCATTCTGTC Chr19:6399374..6399393 60.41 50
upstream ENSMUSE00000695745 Chr19:6399456..6399517 No primer for this exon
upstream ENSMUSE00000695729 Chr19:6399746..6399811 No primer for this exon
upstream ENSMUSE00000695741 Chr19:6400113..6401863 TGGAGATGCACTGTCTTTGC Chr19:6400503..6400522 59.99 50
upstream ENSMUSE00000499968 Chr19:6400562..6400651 TGAACTGCATTCAGGTTTGC Chr19:6400611..6400630 59.85 45
upstream ENSMUSE00000695727 Chr19:6400623..6400651 No primer for this exon

*** Putative Vector Insertion (Chr 19: 6401701 - 6401864) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000695736 Chr19:6401702..6401863 AGGTCCAGAGTGCTCGTCAT Chr19:6401813..6401832 59.87 55
downstream ENSMUSE00000413610 Chr19:6401716..6401863 AGGTCCAGAGTGCTCGTCAT Chr19:6401813..6401832 59.87 55
downstream ENSMUSE00000695732 Chr19:6401718..6401863 AGGTCCAGAGTGCTCGTCAT Chr19:6401813..6401832 59.87 55
downstream ENSMUSE00000695760 Chr19:6401721..6401863 AGGTCCAGAGTGCTCGTCAT Chr19:6401813..6401832 59.87 55
downstream ENSMUSE00000235796 Chr19:6402472..6402574 TGAGAAACATGCGCACTAGC Chr19:6402522..6402541 60.17 50
downstream ENSMUSE00000695731 Chr19:6402552..6402574 No primer for this exon
downstream ENSMUSE00000695730 Chr19:6403024..6403086 No primer for this exon
downstream ENSMUSE00000695804 Chr19:6403024..6403086 No primer for this exon
downstream ENSMUSE00000551256 Chr19:6403031..6403086 No primer for this exon
downstream ENSMUSE00000235751 Chr19:6403432..6403563 GGGTTCAAGTCGAACTCTGC Chr19:6403476..6403495 59.85 55
downstream ENSMUSE00000695726 Chr19:6403432..6403612 GGGTTCAAGTCGAACTCTGC Chr19:6403476..6403495 59.85 55
downstream ENSMUSE00000695728 Chr19:6403432..6403485 GGGTTCAAGTCGAACTCTGC Chr19:6403476..6403495 59.85 55
downstream ENSMUSE00000695739 Chr19:6403432..6403638 GGGTTCAAGTCGAACTCTGC Chr19:6403476..6403495 59.85 55
downstream ENSMUSE00000695744 Chr19:6403432..6403633 GGGTTCAAGTCGAACTCTGC Chr19:6403476..6403495 59.85 55
downstream ENSMUSE00000708058 Chr19:6403432..6403638 GGGTTCAAGTCGAACTCTGC Chr19:6403476..6403495 59.85 55
downstream ENSMUSE00000235727 Chr19:6404300..6404450 ACAAGGACATCTTGCGCTTT Chr19:6404376..6404395 59.88 45
downstream ENSMUSE00000235706 Chr19:6404654..6404827 GAGATGAATCGCTCCAGGAC Chr19:6404727..6404746 59.77 55
downstream ENSMUSE00000235684 Chr19:6404938..6405054 GTCTCCTTGAGGCGTGAGAT Chr19:6405026..6405045 59.41 55
downstream ENSMUSE00000235665 Chr19:6407041..6407322 GAAAAGCTGCCTCATCTTGG Chr19:6407244..6407263 59.96 50
downstream ENSMUSE00000235644 Chr19:6408171..6408248 No primer for this exon
downstream ENSMUSE00000235623 Chr19:6408448..6408570 AGCTTAGGCTTGGCAACTGA Chr19:6408530..6408549 60.15 50
downstream ENSMUSE00000235602 Chr19:6408839..6408954 AGGCGCTGAGATAAGGGAAG Chr19:6408935..6408954 60.86 55
downstream ENSMUSE00000488493 Chr19:6413085..6413226 CCTGGAAGTTGTGTACGAAGC Chr19:6413181..6413201 59.79 52.38
downstream ENSMUSE00000487489 Chr19:6413428..6413464 TGCTTGTAGATGCCCAGGAT Chr19:6413450..6413469 60.62 50
downstream ENSMUSE00000490361 Chr19:6413865..6414041 GTGGCAGTTCACACCACAAG Chr19:6413887..6413906 60.2 55
downstream ENSMUSE00000466930 Chr19:6414792..6414854 CACCTCCTCTTCACGGATCT Chr19:6414814..6414833 59.25 55
downstream ENSMUSE00000466422 Chr19:6414994..6415212 ATTCGGGCCTTTTTATTCCA Chr19:6415204..6415223 60.62 40
downstream ENSMUSE00000695755 Chr19:6414994..6415210 ATTCGGGCCTTTTTATTCCA Chr19:6415204..6415223 60.62 40

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACTCCCACTTCTCCCAGGAC Chr19:6401700..6401720 60.5 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTCTCGTGACTGGGAAAACC Chr19:6401748..6401768 59.7 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000032946