Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI22572
Trapped Gene
Arid5b (ENSMUSG00000019947)
Vector Insertion
Chr 10: 67649005 - 67705751
Public Clones CMHD-GT_483B9-3 (cmhd) IST12320H5 (tigm) IST12139A10 (tigm)
Private Clones OST291355 (lexicon) OST196601 (lexicon) OST103552 (lexicon)
Severity of mutation (?) Insertion after 14% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000289228 (Chr10:67705752..67705977 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000289228 (Chr10:67705752..67705977 -)
Downstram Exon
ENSMUSE00000099301 (Chr10:67648774..67649004 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000666206 Chr10:67741438..67741474 No primer for this exon
upstream ENSMUSE00000393468 Chr10:67740678..67740932 No primer for this exon
upstream ENSMUSE00000289228 Chr10:67705752..67705977 No primer for this exon

*** Putative Vector Insertion (Chr 10: 67649005 - 67705751) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000099301 Chr10:67648774..67649004 No primer for this exon
downstream ENSMUSE00000099314 Chr10:67597742..67597854 No primer for this exon
downstream ENSMUSE00000099312 Chr10:67591537..67591741 No primer for this exon
downstream ENSMUSE00000099319 Chr10:67589447..67589499 No primer for this exon
downstream ENSMUSE00000099321 Chr10:67580999..67581096 No primer for this exon
downstream ENSMUSE00000099298 Chr10:67564643..67564841 No primer for this exon
downstream ENSMUSE00000575802 Chr10:67558341..67561417 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATAATCGCCTTGCAGCACAT Chr10:67705681..67705701 60.64 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGGGACCAAAGAAAGGAAAC Chr10:67705775..67705795 59.78 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TGGACATGGAACAGAGCTGA Chr10:67705952..67705972 60.4 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 ACCAACGTGATTGTGCTCAG Chr10:67705966..67705986 59.75 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000019947