Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI22606
Trapped Gene
Pdcd2l (
ENSMUSG00000002635
)
Vector Insertion
Chr 7: 34969654 - 34969831
Public Clones
not available
Private Clones
OST290435 (lexicon)
Severity of mutation
(?)
Insertion after 88% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000384650 (Chr7:34969655..34969830 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000384650 (Chr7:34969655..34969830 -)
Downstram Exon
ENSMUSE00000675497 (Chr7:34969655..34969830 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000238509
Chr7:34981555..34981669
No primer for this exon
upstream
ENSMUSE00000238500
Chr7:34981291..34981457
No primer for this exon
upstream
ENSMUSE00000238490
Chr7:34981085..34981145
No primer for this exon
upstream
ENSMUSE00000199875
Chr7:34977755..34978125
No primer for this exon
upstream
ENSMUSE00000199873
Chr7:34974368..34974475
No primer for this exon
upstream
ENSMUSE00000199872
Chr7:34971313..34971461
No primer for this exon
upstream
ENSMUSE00000384650
Chr7:34969655..34969830
No primer for this exon
upstream
ENSMUSE00000675497
Chr7:34969655..34969830
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
GAGTAATCGCCTTGCAGCAC
Chr7:34969763..34969783
60.94
55
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
CAGAGCGTGACTGGGAAAAC
Chr7:34969765..34969785
60.83
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000002635
