Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI22659
Trapped Gene
Centa2 (ENSMUSG00000020709)
Vector Insertion
Chr 11: 79987653 - 79989557
Public Clones IST13749E3 (tigm) IST11227C9 (tigm)
Private Clones OST288707 (lexicon) OST148772 (lexicon)
Severity of mutation (?) Insertion after 70% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000108356 (Chr11:79987590..79987652 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000108356 (Chr11:79987590..79987652 +)
Downstram Exon
ENSMUSE00000108353 (Chr11:79989558..79989635 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000349664 Chr11:79967613..79967811 No primer for this exon
upstream ENSMUSE00000283569 Chr11:79968492..79968622 No primer for this exon
upstream ENSMUSE00000108355 Chr11:79970447..79970538 No primer for this exon
upstream ENSMUSE00000108361 Chr11:79973665..79973744 No primer for this exon
upstream ENSMUSE00000108363 Chr11:79975650..79975762 No primer for this exon
upstream ENSMUSE00000108358 Chr11:79979175..79979321 No primer for this exon
upstream ENSMUSE00000108360 Chr11:79984180..79984263 No primer for this exon
upstream ENSMUSE00000108356 Chr11:79987590..79987652 No primer for this exon

*** Putative Vector Insertion (Chr 11: 79987653 - 79989557) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000108353 Chr11:79989558..79989635 No primer for this exon
downstream ENSMUSE00000373060 Chr11:79990548..79990776 No primer for this exon
downstream ENSMUSE00000332693 Chr11:79991898..79992417 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCTCAAACAAGGCTTCATGG Chr11:79987617..79987637 60.63 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCTCAAACAAGGCTTCATGG Chr11:79987617..79987637 60.63 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000020709