Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI22686
Trapped Gene
Prr6 (ENSMUSG00000018509)
Vector Insertion
Chr 11: 62347457 - 62349783
Public Clones not available
Private Clones OST287913 (lexicon) OST251924 (lexicon) OST238153 (lexicon) OST66216 (lexicon)
OST50839 (lexicon) OST44180 (lexicon) OST39305 (lexicon)
Severity of mutation (?) Insertion after 59% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000106582 (Chr11:62349784..62349882 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000106582 (Chr11:62349784..62349882 -)
Downstram Exon
ENSMUSE00000106583 (Chr11:62347387..62347456 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000335488 Chr11:62352358..62352763 No primer for this exon
upstream ENSMUSE00000106582 Chr11:62349784..62349882 No primer for this exon

*** Putative Vector Insertion (Chr 11: 62347457 - 62349783) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000106583 Chr11:62347387..62347456 No primer for this exon
downstream ENSMUSE00000106584 Chr11:62340994..62341108 No primer for this exon
downstream ENSMUSE00000106587 Chr11:62338448..62338789 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TGGGCTTCAGCAGACTTACA Chr11:62349797..62349817 59.59 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGTTTGGGCTTCAGCAGACT Chr11:62349801..62349821 59.09 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000018509