Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI22822
Trapped Gene
Tln1 (ENSMUSG00000028465)
Vector Insertion
Chr 4: 43568726 - 43568857
Public Clones not available
Private Clones OST283951 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000179473 (Chr4:43568727..43568856 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CAAGACCGTCACAGACATGC Chr4:43568751..43568770 60.32 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000179473 (Chr4:43568727..43568856 -)
Downstram Exon
ENSMUSE00000674763 (Chr4:43568727..43568856 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CAAGACCGTCACAGACATGC Chr4:43568751..43568770 60.32 55 CTGTGACGGTCTTGGAGTCA Chr4:43568735..43568754 59.86 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000289986 Chr4:43575002..43575127 GAAGTGGCCGAGAGTGTGTC Chr4:43575091..43575110 60.87 60
upstream ENSMUSE00000711522 Chr4:43569493..43569655 ATGTGGTGAAGACGATGCAA Chr4:43569569..43569588 60.12 45
upstream ENSMUSE00000716731 Chr4:43569493..43569655 ATGTGGTGAAGACGATGCAA Chr4:43569569..43569588 60.12 45
upstream ENSMUSE00000179477 Chr4:43569161..43569258 CAACGACTTTGGGCTCTTTC Chr4:43569238..43569257 59.85 50
upstream ENSMUSE00000674764 Chr4:43569161..43569258 CAACGACTTTGGGCTCTTTC Chr4:43569238..43569257 59.85 50
upstream ENSMUSE00000179473 Chr4:43568727..43568856 CAAGACCGTCACAGACATGC Chr4:43568751..43568770 60.32 55
upstream ENSMUSE00000674763 Chr4:43568727..43568856 CAAGACCGTCACAGACATGC Chr4:43568751..43568770 60.32 55

*** Putative Vector Insertion (Chr 4: 43568726 - 43568857) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000179429 Chr4:43568474..43568626 CTTCCATCAGCTCTCGAACC Chr4:43568557..43568576 59.95 55
downstream ENSMUSE00000674762 Chr4:43568474..43568626 CTTCCATCAGCTCTCGAACC Chr4:43568557..43568576 59.95 55
downstream ENSMUSE00000179459 Chr4:43568141..43568283 AAGAACTTTCTCCGCAGCAG Chr4:43568180..43568199 59.76 50
downstream ENSMUSE00000674761 Chr4:43568141..43568283 AAGAACTTTCTCCGCAGCAG Chr4:43568180..43568199 59.76 50
downstream ENSMUSE00000179446 Chr4:43567919..43568046 ACAGGCCTTGTCAAAGGAGA Chr4:43567971..43567990 59.84 50
downstream ENSMUSE00000674760 Chr4:43567919..43568046 ACAGGCCTTGTCAAAGGAGA Chr4:43567971..43567990 59.84 50
downstream ENSMUSE00000179474 Chr4:43567150..43567210 TCTTACGCTCTCCCTTCTGC Chr4:43567136..43567155 59.72 55
downstream ENSMUSE00000674759 Chr4:43567150..43567210 TCTTACGCTCTCCCTTCTGC Chr4:43567136..43567155 59.72 55
downstream ENSMUSE00000527570 Chr4:43566318..43566422 TAGCGTACCTTGGCCTCAAT Chr4:43566351..43566370 59.73 50
downstream ENSMUSE00000674758 Chr4:43566318..43566422 TAGCGTACCTTGGCCTCAAT Chr4:43566351..43566370 59.73 50
downstream ENSMUSE00000527569 Chr4:43565858..43566013 ATCCACACGCATCACACACT Chr4:43565917..43565936 60.04 50
downstream ENSMUSE00000674757 Chr4:43565858..43566013 ATCCACACGCATCACACACT Chr4:43565917..43565936 60.04 50
downstream ENSMUSE00000179439 Chr4:43564058..43564159 CTCGCCTTCTGTCGTCTGTA Chr4:43564084..43564103 59.19 55
downstream ENSMUSE00000179441 Chr4:43563834..43563910 GGGTGACACTGAGTCCTCCA Chr4:43563817..43563836 61.15 60
downstream ENSMUSE00000179442 Chr4:43563070..43563250 TTTCCCCACTCGGTTGTACT Chr4:43563189..43563208 59.45 50
downstream ENSMUSE00000179490 Chr4:43562810..43562923 CTGCATGCTGGAGTTAATGG Chr4:43562854..43562873 59.3 50
downstream ENSMUSE00000179436 Chr4:43562595..43562703 GGGAGTGGATCTCATGCTTT Chr4:43562624..43562643 59.09 50
downstream ENSMUSE00000179470 Chr4:43562193..43562401 ATCTCCGTCAGGTTGGAAGA Chr4:43562307..43562326 59.65 50
downstream ENSMUSE00000179455 Chr4:43561958..43562056 GTGGGGGTCAGTGTCACTTT Chr4:43561942..43561961 59.86 55
downstream ENSMUSE00000674756 Chr4:43560995..43561018 No primer for this exon
downstream ENSMUSE00000179453 Chr4:43560851..43561018 TAGCTGCAGCAATCACTTGG Chr4:43560873..43560892 60.16 50
downstream ENSMUSE00000179466 Chr4:43560374..43560668 TGGTATCAGTGGCTTGGTCA Chr4:43560397..43560416 60.11 50
downstream ENSMUSE00000674755 Chr4:43560374..43560473 TGGTATCAGTGGCTTGGTCA Chr4:43560397..43560416 60.11 50
downstream ENSMUSE00000674773 Chr4:43560374..43560518 TGGTATCAGTGGCTTGGTCA Chr4:43560397..43560416 60.11 50
downstream ENSMUSE00000674772 Chr4:43559724..43559788 AGCATTGACCAGGTCTGAGG Chr4:43559708..43559727 60.26 55
downstream ENSMUSE00000718914 Chr4:43559622..43559788 GCATTGACCAGGTCTGAGGT Chr4:43559709..43559728 60.12 55
downstream ENSMUSE00000721103 Chr4:43559622..43559788 GCATTGACCAGGTCTGAGGT Chr4:43559709..43559728 60.12 55
downstream ENSMUSE00000179448 Chr4:43558732..43558860 CGCTGCACCAACTTCTTCTT Chr4:43558717..43558736 60.57 50
downstream ENSMUSE00000674753 Chr4:43558732..43558860 CGCTGCACCAACTTCTTCTT Chr4:43558717..43558736 60.57 50
downstream ENSMUSE00000179444 Chr4:43558478..43558594 CTGCAGCAATGGTCTGTGTT Chr4:43558524..43558543 59.9 50
downstream ENSMUSE00000674752 Chr4:43558478..43558594 CTGCAGCAATGGTCTGTGTT Chr4:43558524..43558543 59.9 50
downstream ENSMUSE00000674771 Chr4:43558478..43558519 TACAGCTCTGCACCAGTAGGG Chr4:43558458..43558478 60.46 57.14
downstream ENSMUSE00000660844 Chr4:43558279..43558392 GAGCTTGACTTCCTCGGACA Chr4:43558316..43558335 60.53 55
downstream ENSMUSE00000710922 Chr4:43558279..43558392 GAGCTTGACTTCCTCGGACA Chr4:43558316..43558335 60.53 55
downstream ENSMUSE00000720893 Chr4:43558279..43558392 GAGCTTGACTTCCTCGGACA Chr4:43558316..43558335 60.53 55
downstream ENSMUSE00000179464 Chr4:43557966..43558100 GGTCCTGAATTGTTGGCACT Chr4:43558024..43558043 59.97 50
downstream ENSMUSE00000674769 Chr4:43557966..43558100 GGTCCTGAATTGTTGGCACT Chr4:43558024..43558043 59.97 50
downstream ENSMUSE00000179438 Chr4:43557713..43557841 CCATCTCCAAAGGTCCACAT Chr4:43557789..43557808 59.78 50
downstream ENSMUSE00000674767 Chr4:43557713..43557841 CCATCTCCAAAGGTCCACAT Chr4:43557789..43557808 59.78 50
downstream ENSMUSE00000179472 Chr4:43557448..43557550 TTTGGTGCTGTTGCCAAGAT Chr4:43557490..43557509 61.61 45
downstream ENSMUSE00000674766 Chr4:43557448..43557550 TTTGGTGCTGTTGCCAAGAT Chr4:43557490..43557509 61.61 45
downstream ENSMUSE00000674765 Chr4:43557175..43557360 CTGGCTGTGTCAAGCACAAT Chr4:43557224..43557243 59.9 50
downstream ENSMUSE00000179430 Chr4:43557152..43557360 CTGGCTGTGTCAAGCACAAT Chr4:43557224..43557243 59.9 50
downstream ENSMUSE00000674750 Chr4:43557152..43557360 CTGGCTGTGTCAAGCACAAT Chr4:43557224..43557243 59.9 50
downstream ENSMUSE00000179481 Chr4:43556689..43556808 CGTAGGGCATTATCCACGTC Chr4:43556707..43556726 60.35 55
downstream ENSMUSE00000674749 Chr4:43556689..43556808 CGTAGGGCATTATCCACGTC Chr4:43556707..43556726 60.35 55
downstream ENSMUSE00000179451 Chr4:43556308..43556505 TTCATTCAATCGGCTCTGTG Chr4:43556433..43556452 59.8 45
downstream ENSMUSE00000674748 Chr4:43556308..43556505 TTCATTCAATCGGCTCTGTG Chr4:43556433..43556452 59.8 45
downstream ENSMUSE00000179433 Chr4:43556016..43556155 GATATGCCCTTCAGGTTGGA Chr4:43556087..43556106 59.89 50
downstream ENSMUSE00000674747 Chr4:43556016..43556155 GATATGCCCTTCAGGTTGGA Chr4:43556087..43556106 59.89 50
downstream ENSMUSE00000179463 Chr4:43555747..43555840 GTTGATGCTGTCCGTCACTG Chr4:43555797..43555816 60.32 55
downstream ENSMUSE00000674746 Chr4:43555747..43555840 GTTGATGCTGTCCGTCACTG Chr4:43555797..43555816 60.32 55
downstream ENSMUSE00000179435 Chr4:43555574..43555663 TCCAGGAGTTCTCGGACTGT Chr4:43555622..43555641 59.83 55
downstream ENSMUSE00000674744 Chr4:43555574..43555663 TCCAGGAGTTCTCGGACTGT Chr4:43555622..43555641 59.83 55
downstream ENSMUSE00000179479 Chr4:43555353..43555475 CAATGGCATCTCCAAACTCC Chr4:43555378..43555397 60.46 50
downstream ENSMUSE00000674743 Chr4:43555353..43555475 CAATGGCATCTCCAAACTCC Chr4:43555378..43555397 60.46 50
downstream ENSMUSE00000179488 Chr4:43553402..43553545 GTTCCACCAGTCCTTGCTGT Chr4:43553460..43553479 60.16 55
downstream ENSMUSE00000674742 Chr4:43553402..43553545 GTTCCACCAGTCCTTGCTGT Chr4:43553460..43553479 60.16 55
downstream ENSMUSE00000179469 Chr4:43552848..43553009 AGGCGACAGCTGTTACACAA Chr4:43552926..43552945 59.51 50
downstream ENSMUSE00000674740 Chr4:43552848..43553009 AGGCGACAGCTGTTACACAA Chr4:43552926..43552945 59.51 50
downstream ENSMUSE00000179485 Chr4:43552509..43552649 CTGGAGAACTCAGGGTTGGA Chr4:43552512..43552531 60.23 55
downstream ENSMUSE00000674739 Chr4:43552509..43552649 CTGGAGAACTCAGGGTTGGA Chr4:43552512..43552531 60.23 55
downstream ENSMUSE00000179482 Chr4:43552238..43552419 GTCTCGGGGATTGACTGCTA Chr4:43552293..43552312 60.22 55
downstream ENSMUSE00000674738 Chr4:43552238..43552419 GTCTCGGGGATTGACTGCTA Chr4:43552293..43552312 60.22 55
downstream ENSMUSE00000179487 Chr4:43551963..43552095 AATGGCTGTCTCACACTCCA Chr4:43552034..43552053 59.26 50
downstream ENSMUSE00000674737 Chr4:43551963..43552095 AATGGCTGTCTCACACTCCA Chr4:43552034..43552053 59.26 50
downstream ENSMUSE00000179458 Chr4:43551550..43551651 CGGCTCAATGAGATGAGAAA Chr4:43551573..43551592 58.95 45
downstream ENSMUSE00000674735 Chr4:43551550..43551651 CGGCTCAATGAGATGAGAAA Chr4:43551573..43551592 58.95 45
downstream ENSMUSE00000179484 Chr4:43551304..43551474 CCAGGGTGAGTGGTTCAAAG Chr4:43551413..43551432 60.54 55
downstream ENSMUSE00000674733 Chr4:43551304..43551474 CCAGGGTGAGTGGTTCAAAG Chr4:43551413..43551432 60.54 55
downstream ENSMUSE00000179462 Chr4:43551062..43551211 CAGGTCTTCTACGGCCTCTG Chr4:43551121..43551140 60.01 60
downstream ENSMUSE00000674732 Chr4:43551062..43551211 CAGGTCTTCTACGGCCTCTG Chr4:43551121..43551140 60.01 60
downstream ENSMUSE00000179461 Chr4:43550858..43550959 GGGTCACCCATAGGTCCTTC Chr4:43550912..43550931 60.58 60
downstream ENSMUSE00000674731 Chr4:43550858..43550959 GGGTCACCCATAGGTCCTTC Chr4:43550912..43550931 60.58 60
downstream ENSMUSE00000179471 Chr4:43550660..43550779 AGAGGGCCTAGCTCTTCAGG Chr4:43550717..43550736 60.11 60
downstream ENSMUSE00000674730 Chr4:43550660..43550779 AGAGGGCCTAGCTCTTCAGG Chr4:43550717..43550736 60.11 60
downstream ENSMUSE00000179447 Chr4:43549396..43549542 GTGTTTGATGTGAGCGCCTA Chr4:43549500..43549519 59.87 50
downstream ENSMUSE00000674728 Chr4:43549396..43549542 GTGTTTGATGTGAGCGCCTA Chr4:43549500..43549519 59.87 50
downstream ENSMUSE00000179475 Chr4:43549116..43549282 CAGCACCCTCACGGTTAAGT Chr4:43549113..43549132 60.17 55
downstream ENSMUSE00000674727 Chr4:43549116..43549282 CAGCACCCTCACGGTTAAGT Chr4:43549113..43549132 60.17 55
downstream ENSMUSE00000289719 Chr4:43548825..43549008 CAGCTGCATTCTGCACTAGG Chr4:43548919..43548938 59.76 55
downstream ENSMUSE00000674726 Chr4:43548825..43549008 CAGCTGCATTCTGCACTAGG Chr4:43548919..43548938 59.76 55
downstream ENSMUSE00000289709 Chr4:43548610..43548735 ACTGCATTGATCAGCACCAC Chr4:43548694..43548713 59.71 50
downstream ENSMUSE00000674725 Chr4:43548610..43548735 ACTGCATTGATCAGCACCAC Chr4:43548694..43548713 59.71 50
downstream ENSMUSE00000354369 Chr4:43548392..43548508 CGTATGTGCTCTGTGGTTGC Chr4:43548383..43548402 60.33 55
downstream ENSMUSE00000674724 Chr4:43548392..43548508 CGTATGTGCTCTGTGGTTGC Chr4:43548383..43548402 60.33 55
downstream ENSMUSE00000413328 Chr4:43547452..43547637 TTCAGGGGTAGAGGTCTTGG Chr4:43547571..43547590 59.13 55
downstream ENSMUSE00000674723 Chr4:43547452..43547637 TTCAGGGGTAGAGGTCTTGG Chr4:43547571..43547590 59.13 55
downstream ENSMUSE00000372500 Chr4:43547262..43547369 GTACATCAGGCGCCACTTCT Chr4:43547311..43547330 60.28 55
downstream ENSMUSE00000674722 Chr4:43547262..43547369 GTACATCAGGCGCCACTTCT Chr4:43547311..43547330 60.28 55
downstream ENSMUSE00000403999 Chr4:43546735..43546840 TCCAGTTAGCTGCTGCTTCA Chr4:43546774..43546793 59.89 50
downstream ENSMUSE00000674721 Chr4:43546735..43546840 TCCAGTTAGCTGCTGCTTCA Chr4:43546774..43546793 59.89 50
downstream ENSMUSE00000179486 Chr4:43546426..43546550 TTCAGCTGCTCCAGCTTCTT Chr4:43546423..43546442 60.42 50
downstream ENSMUSE00000674719 Chr4:43546426..43546550 TTCAGCTGCTCCAGCTTCTT Chr4:43546423..43546442 60.42 50
downstream ENSMUSE00000179457 Chr4:43546176..43546301 CTGACGCAGCCTTTACCAGT Chr4:43546186..43546205 60.45 55
downstream ENSMUSE00000674718 Chr4:43546176..43546301 CTGACGCAGCCTTTACCAGT Chr4:43546186..43546205 60.45 55
downstream ENSMUSE00000179460 Chr4:43545998..43546060 ACCACTGCCCATCATCCAGT Chr4:43545996..43546015 62.76 55
downstream ENSMUSE00000674717 Chr4:43545998..43546060 ACCACTGCCCATCATCCAGT Chr4:43545996..43546015 62.76 55
downstream ENSMUSE00000179428 Chr4:43545702..43545884 CTGCGTTAGCTGCTTCACAC Chr4:43545811..43545830 59.81 55
downstream ENSMUSE00000674716 Chr4:43545702..43545884 CTGCGTTAGCTGCTTCACAC Chr4:43545811..43545830 59.81 55
downstream ENSMUSE00000179450 Chr4:43545064..43545192 CACCACCGTCTCATTCTCCT Chr4:43545075..43545094 60.11 55
downstream ENSMUSE00000674715 Chr4:43545064..43545192 CACCACCGTCTCATTCTCCT Chr4:43545075..43545094 60.11 55
downstream ENSMUSE00000347950 Chr4:43544393..43544957 TGGGTTTACAACCCTCAAGC Chr4:43544401..43544420 59.97 50
downstream ENSMUSE00000660843 Chr4:43544393..43544957 TGGGTTTACAACCCTCAAGC Chr4:43544401..43544420 59.97 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGATGTTAATCGCCTTGCAG Chr4:43568792..43568812 60.61 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATGTCGTGACTGGGAAAACC Chr4:43568790..43568810 59.83 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000028465