Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI22920
Trapped Gene
Usp12 (ENSMUSG00000029640)
Vector Insertion
Chr 5: 147565945 - 147566176
Public Clones not available
Private Clones OST281170 (lexicon) OST201473 (lexicon) OST144662 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000589480 (Chr5:147565946..147566175 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GGTCCACGAGATCTTTCAGG Chr5:147565987..147566006 59.65 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000589480 (Chr5:147565946..147566175 -)
Downstram Exon
ENSMUSE00000190971 (Chr5:147565946..147566175 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GGTCCACGAGATCTTTCAGG Chr5:147565987..147566006 59.65 55 CCCTGAAAGATCTCGTGGAC Chr5:147565964..147565983 59.65 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000711990 Chr5:147606191..147606532 TTCGCCTCCATCTGTACCAT Chr5:147606192..147606211 60.48 50
upstream ENSMUSE00000714055 Chr5:147606191..147606532 TTCGCCTCCATCTGTACCAT Chr5:147606192..147606211 60.48 50
upstream ENSMUSE00000376315 Chr5:147580513..147580593 AGAACAGTTTCCGGTCAACG Chr5:147580536..147580555 60.15 50
upstream ENSMUSE00000589481 Chr5:147580513..147580593 CCGGTCAACGAGCACTATTT Chr5:147580526..147580545 60.13 50
upstream ENSMUSE00000263651 Chr5:147574777..147574990 ACAAGGTTGCGGAAAGAAAA Chr5:147574779..147574798 59.72 40
upstream ENSMUSE00000486429 Chr5:147574777..147574990 ACAAGGTTGCGGAAAGAAAA Chr5:147574779..147574798 59.72 40
upstream ENSMUSE00000190971 Chr5:147565946..147566175 CTGGGTCCACGAGATCTTTC Chr5:147565990..147566009 59.65 55
upstream ENSMUSE00000589480 Chr5:147565946..147566175 GGTCCACGAGATCTTTCAGG Chr5:147565987..147566006 59.65 55

*** Putative Vector Insertion (Chr 5: 147565945 - 147566176) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000647754 Chr5:147563469..147563545 TGTGTTTTGTTCCACGTCAA Chr5:147563467..147563486 58.57 40
downstream ENSMUSE00000647753 Chr5:147563271..147563354 CTCCGGCACTCTTCACAGTA Chr5:147563270..147563289 59.03 55
downstream ENSMUSE00000533711 Chr5:147551878..147552067 GCATCTCCTGACGTGTTGAA Chr5:147551902..147551921 59.84 50
downstream ENSMUSE00000263626 Chr5:147551870..147552067 GCATCTCCTGACGTGTTGAA Chr5:147551902..147551921 59.84 50
downstream ENSMUSE00000647752 Chr5:147551870..147551875 No primer for this exon
downstream ENSMUSE00000533709 Chr5:147550676..147550754 AATATAATGGCCTCGGTTGG Chr5:147550708..147550727 58.78 45
downstream ENSMUSE00000533718 Chr5:147550676..147550754 AATATAATGGCCTCGGTTGG Chr5:147550708..147550727 58.78 45
downstream ENSMUSE00000510269 Chr5:147546388..147549356 AACGACAATCCCCTGAAGTG Chr5:147547013..147547032 59.97 50
downstream ENSMUSE00000647751 Chr5:147546388..147549356 AACGACAATCCCCTGAAGTG Chr5:147547013..147547032 59.97 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCAACAAGATGCCCATGAAT Chr5:147566135..147566155 60.87 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCAACAAGATGCCCATGAAT Chr5:147566135..147566155 60.87 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000029640