Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI23040
Trapped Gene
Slc7a7 (ENSMUSG00000000958)
Vector Insertion
Chr 14: 55027886 - 55028086
Public Clones P124B10 (ggtc) IST14965G9 (tigm) IST14522B9 (tigm) IST14919D2 (tigm)
IST14683D10 (tigm)
Private Clones OST278400 (lexicon) OST214622 (lexicon) OST205139 (lexicon) OST185200 (lexicon)
OST146353 (lexicon) OST58677 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000323914 (Chr14:55028087..55028297 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000323914 (Chr14:55028087..55028297 -)
Downstram Exon
ENSMUSE00000124136 (Chr14:55027340..55027885 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000323914 Chr14:55028087..55028297 No primer for this exon

*** Putative Vector Insertion (Chr 14: 55027886 - 55028086) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000124136 Chr14:55027340..55027885 No primer for this exon
downstream ENSMUSE00000124133 Chr14:54998784..54998909 No primer for this exon
downstream ENSMUSE00000124135 Chr14:54997450..54997594 No primer for this exon
downstream ENSMUSE00000559616 Chr14:54994232..54994355 No primer for this exon
downstream ENSMUSE00000124130 Chr14:54993898..54994001 No primer for this exon
downstream ENSMUSE00000124131 Chr14:54993526..54993622 No primer for this exon
downstream ENSMUSE00000124128 Chr14:54992624..54992773 No primer for this exon
downstream ENSMUSE00000124132 Chr14:54989777..54989960 No primer for this exon
downstream ENSMUSE00000388042 Chr14:54989128..54989570 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTGTGGGGGAAAAGTAATCG Chr14:55028029..55028049 59.79 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGGTTGAGGGTTCAGCTGTC Chr14:55028096..55028116 59.31 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 CCTTAGGGGAAAGAACAGCA Chr14:55028292..55028312 59.31 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 CCTTAGGGGAAAGAACAGCA Chr14:55028292..55028312 59.31 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000000958