Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI23113
Trapped Gene
Zfp185 (ENSMUSG00000031351)
Vector Insertion
Chr X: 70242373 - 70242452
Public Clones not available
Private Clones OST275996 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000720960 (ChrX:70242374..70242451 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000720960 (ChrX:70242374..70242451 +)
Downstram Exon
ENSMUSE00000708297 (ChrX:70242374..70242451 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000699738 ChrX:70232721..70232858 No primer for this exon

*** Putative Vector Insertion (Chr X: 70242373 - 70242452) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000708297 ChrX:70242374..70242451 No primer for this exon
downstream ENSMUSE00000720960 ChrX:70242374..70242451 No primer for this exon
downstream ENSMUSE00000699714 ChrX:70242621..70242744 CATCATGCTTGGTGATCCAG ChrX:70242728..70242747 60.07 50
downstream ENSMUSE00000699736 ChrX:70242621..70242744 CATCATGCTTGGTGATCCAG ChrX:70242728..70242747 60.07 50
downstream ENSMUSE00000699712 ChrX:70243077..70243142 TGGCATTTGATTCGTCTCTG ChrX:70243144..70243163 59.8 45
downstream ENSMUSE00000699735 ChrX:70243077..70243142 TGGCATTTGATTCGTCTCTG ChrX:70243144..70243163 59.8 45
downstream ENSMUSE00000699704 ChrX:70243121..70243142 TGGCATTTGATTCGTCTCTG ChrX:70243144..70243163 59.8 45
downstream ENSMUSE00000699711 ChrX:70243875..70243913 No primer for this exon
downstream ENSMUSE00000699734 ChrX:70243875..70243913 No primer for this exon
downstream ENSMUSE00000699710 ChrX:70244008..70244085 TGGAGCTCCGTTGGTCTTAG ChrX:70244083..70244102 60.39 55
downstream ENSMUSE00000699732 ChrX:70244008..70244085 TGGAGCTCCGTTGGTCTTAG ChrX:70244083..70244102 60.39 55
downstream ENSMUSE00000655386 ChrX:70244590..70244676 AGCCAGACGACTGAAGAGGA ChrX:70244648..70244667 60.13 55
downstream ENSMUSE00000699731 ChrX:70244590..70244676 AGCCAGACGACTGAAGAGGA ChrX:70244648..70244667 60.13 55
downstream ENSMUSE00000715521 ChrX:70244590..70244676 AGCCAGACGACTGAAGAGGA ChrX:70244648..70244667 60.13 55
downstream ENSMUSE00000208571 ChrX:70245356..70245439 GTGAATGGCACCTCCTCTTC ChrX:70245421..70245440 59.66 55
downstream ENSMUSE00000274297 ChrX:70246327..70246410 CCTCAGAACACCAAGTGCAG ChrX:70246357..70246376 59.46 55
downstream ENSMUSE00000208566 ChrX:70246744..70246785 No primer for this exon
downstream ENSMUSE00000699730 ChrX:70246747..70246785 TTTTGCAAGGAATGGCGACT ChrX:70246786..70246805 62.92 45
downstream ENSMUSE00000208560 ChrX:70247914..70247997 AGCAGGTGGTTCCTGGTTTT ChrX:70247971..70247990 60.91 50
downstream ENSMUSE00000274276 ChrX:70248712..70248798 ACCAGGTGGAGTTCTGCTGT ChrX:70248777..70248796 59.76 55
downstream ENSMUSE00000274267 ChrX:70257779..70257967 CATCTTGTAGGGTGCCAGGT ChrX:70257855..70257874 59.99 55
downstream ENSMUSE00000208574 ChrX:70261078..70261188 TGCTTGAGACACCAGGAACA ChrX:70261169..70261188 60.44 50
downstream ENSMUSE00000208587 ChrX:70262034..70262114 TGGCTTCCCAGAAGACACTT ChrX:70262097..70262116 59.84 50
downstream ENSMUSE00000208563 ChrX:70263713..70263781 TACCTTCATGTGGGGCTTTC ChrX:70263771..70263790 59.93 50
downstream ENSMUSE00000208562 ChrX:70266207..70266309 GCAGATGCCAAGATGTTCAA ChrX:70266291..70266310 59.81 45
downstream ENSMUSE00000208585 ChrX:70274184..70274282 GGAGATCACCCATCGGTTTA ChrX:70274220..70274239 59.75 50
downstream ENSMUSE00000699703 ChrX:70275012..70276882 CCAGGGATCGTCAACAGTTT ChrX:70275377..70275396 59.97 50
downstream ENSMUSE00000699719 ChrX:70275012..70276852 CCAGGGATCGTCAACAGTTT ChrX:70275377..70275396 59.97 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CAGGAGCTCACTGAATCCAC ChrX:70242372..70242392 58.38 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031351