Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI23136
Trapped Gene
Il34 (ENSMUSG00000031750)
Vector Insertion
Chr 8: 113273459 - 113279130
Public Clones CMHD-GT_298C10-3 (cmhd) IST14576C10 (tigm)
Private Clones OST275328 (lexicon)
Severity of mutation (?) Insertion after 23% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000466224 (Chr8:113279131..113279550 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ACCAGTTCCTGAGCTGCAAT Chr8:113279378..113279397 59.87 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000466224 (Chr8:113279131..113279550 -)
Downstram Exon
ENSMUSE00000634404 (Chr8:113273325..113273458 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ACCAGTTCCTGAGCTGCAAT Chr8:113279378..113279397 59.87 50 CCCAAAGCCACGTCAAGTAG Chr8:113273406..113273425 60.68 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000463463 Chr8:113329634..113329772 GCGACTGTAGAAGCCCACAT Chr8:113329650..113329669 60.28 55
upstream ENSMUSE00000466224 Chr8:113279131..113279550 ACCAGTTCCTGAGCTGCAAT Chr8:113279378..113279397 59.87 50

*** Putative Vector Insertion (Chr 8: 113273459 - 113279130) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000634404 Chr8:113273325..113273458 CCCAAAGCCACGTCAAGTAG Chr8:113273406..113273425 60.68 55
downstream ENSMUSE00000634403 Chr8:113272563..113272640 GTACCCCCTCATAAGGCACA Chr8:113272567..113272586 59.81 55
downstream ENSMUSE00000634402 Chr8:113272143..113272304 GCCCTCGAGAAGTACATCCA Chr8:113272187..113272206 60.22 55
downstream ENSMUSE00000311661 Chr8:113270718..113270835 GCTGGTGTCAAATGATCTGG Chr8:113270718..113270737 59.09 50
downstream ENSMUSE00000580056 Chr8:113266544..113266679 No primer for this exon
downstream ENSMUSE00000580059 Chr8:113266544..113266679 No primer for this exon
downstream ENSMUSE00000580058 Chr8:113265740..113266348 GCAAGATACGGCATTTGGTT Chr8:113265769..113265788 59.97 45
downstream ENSMUSE00000679407 Chr8:113265740..113266348 GCAAGATACGGCATTTGGTT Chr8:113265769..113265788 59.97 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGTACTGGGCATGGGGAGTT Chr8:113279106..113279126 60.77 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGTACTGGGCATGGGGAGTT Chr8:113279106..113279126 60.77 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TTGATGATGGATGGTGGTTG Chr8:113279533..113279553 60.18 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 TTGATGATGGATGGTGGTTG Chr8:113279533..113279553 60.18 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031750