Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI2322
Trapped Gene
Prcc (ENSMUSG00000004895)
Vector Insertion
Chr 3: 87664134 - 87666052
Public Clones AC0072 (sanger) AE0251 (sanger) (sanger) CMHD-GT_460D8-3 (cmhd)
Private Clones OST337663 (lexicon) OST177090 (lexicon) OST177070 (lexicon)
Severity of mutation (?) Insertion after 90% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000295360 (Chr3:87666053..87666196 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000295360 (Chr3:87666053..87666196 -)
Downstram Exon
ENSMUSE00000356432 (Chr3:87664068..87664133 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000175818 Chr3:87688797..87689503 No primer for this exon
upstream ENSMUSE00000175822 Chr3:87676132..87676179 No primer for this exon
upstream ENSMUSE00000175819 Chr3:87673505..87674071 No primer for this exon
upstream ENSMUSE00000295372 Chr3:87671237..87671332 No primer for this exon
upstream ENSMUSE00000295360 Chr3:87666053..87666196 No primer for this exon

*** Putative Vector Insertion (Chr 3: 87664134 - 87666052) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000356432 Chr3:87664068..87664133 No primer for this exon
downstream ENSMUSE00000465061 Chr3:87662839..87663268 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATTTGGATGGCCTACTTGGA Chr3:87666015..87666035 59.39 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATTTGGATGGCCTACTTGGA Chr3:87666015..87666035 59.39 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 AGAGGAACCGAGGGAGAGAG Chr3:87666156..87666176 59.94 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 AGAGGAACCGAGGGAGAGAG Chr3:87666156..87666176 59.94 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000004895